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RT-LAMP Fluorencent Master Mix HCB5205A Kiʻi Manaʻo
  • RT-LAMP Fluorencent Master Mix HCB5205A

RT-LAMP Fluorencent Master Mix


Helu popoki: HCB5205A

Pūʻolo: 96RXN/960RXN/9600RXN

Aia i loko o kēia huahana ka mea ho'ōla ho'ōla, RT-Enzymes Mix (Bst DNA polymerase and heat-resistant reverse transcriptase), lyophilized Protectants a me nā ʻāpana dye chromogenic.

Hōʻike huahana

kikoʻī huahana

Aia i loko o kēia huahana ka mea ho'ōla ho'ōla, RT-Enzymes Mix (Bst DNA polymerase and heat-resistant reverse transcriptase), lyophilized Protectants aʻulaʻulaʻāpana kala.Aia ka Mg2+, dNTP a me nā mea pono ʻē aʻe no ka hoʻonui.No ka hoʻohana ʻana, hoʻohui wale i ka Buffer, reaction enzyme, fluorescent dye, primer a hoʻohui i ka template, hiki ke hoʻohana pololei i ka pale lyophilized.Ua hoʻohui ʻia i kahi lyophilizer a lyophilized, a ua hoʻohui ʻia nā primers a me nā templates i ka wā i hoʻohana ʻia.Loaʻa i kēia reagent ka pono amplification kiʻekiʻe a me ka naʻau.


  • Mua:
  • Aʻe:

  • ʻāpana

    ʻāpana

    HCB5205A-01

    HCB5205A-02

    HCB5205A-03

    Hoʻopaʻa hoʻonui i hoʻopaʻa ʻia i ka Loop

    1.2 mL

    4 mL×3

    12 mL×10

    Huihui RT-Enzymes

    245 μL

    2.45 mL

    2.45 mL×10

    Lyophilized pale

    0.96 mL×2

    9.60 mL×2

    9.60 mL×20

    25× Waikani

    192 μL

    0.96 mL×2

    0.96 mL×20

     

    Nā noi

    No ka DNA a i ʻole RNA isothermal amplification.

     

    Nā Kūlana Waihona

    Lawe ʻia me ka hau maloʻo, mālama ʻia ma -25~ -15 ℃.E hōʻalo i ka hoʻoheheʻe pinepine ʻana, pono ka huahana no 12 mahina.

     

    Kūkākūkā

    1.E hoʻoheheʻe i ka pale pale e hoʻohana ʻia ma ka wela lumi.Vortex pōkole a hoʻohuli paha i nā paipu i nā manawa he nui e hui pono, a laila centrifuge e hōʻiliʻili i ka wai i lalo o ka paipu.

    2.Hoʻomākaukau i ka ʻōnaehana pane.Hiki ke hoʻomākaukau ʻia kēia reagent i ʻelua ʻōnaehana hopena, ka hui ʻana o ka wai a me ka ʻōnaehana lyophilized.

    1) E hoʻomākaukau i ka hui ʻana o ka wai

    ʻāpana

    Volume

    Hoʻopaʻa hoʻonui i hoʻopaʻa ʻia i ka Loop

    12.5 μL

    25× Waikani

    2 μL

    Huihui RT-Enzymes

    2.55 μL

    10 × Hui Pūmuaa

    5 μL

    Nā templates DNA/RNA b

    × μL

    Wai Nuclease-free

    A hiki i 50 μL

     

    2) Hui pūnaewele Lyophilization

    ① E hoʻomākaukau i ka hui lyophilized

    ʻāpana

    Volume

    Hoʻopaʻa hoʻonui i hoʻopaʻa ʻia i ka Loop

    12.5 μL

    Lyophilized pale

    20 μL

    25× Waikani

    2 μL

    Huihui RT-Enzymes

    2.55 μL

    Wai Nuclease-free

    A hiki i 50 μL

    ② Lyophilization: Ua lyophilized ka Mix i hoʻomākaukau ʻia ma kahi ʻōnaehana 50μL

    ③ E hoʻomākaukau i ka hui ʻana

    ʻāpana

    Volume

    Hoʻohui lyophilized

    1 apana

    10 × Hui Pūmuaa

    5 μL

    Nā templates DNA/RNA b

    × μL

    Wai Nuclease-free

    A hiki i 50 μL

    Nā memo:

    1) a.10×Primer Mix : 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;

    2) b.Paipai ʻia ʻo DEPC (water soluble) no ka templ acid nucleic.

    3)Pane Hoʻonui: Hoʻonohonoho i kēia papahana ma kahi mea hana PCR fluorescent (e laʻa me ABI7500, etc.):

    Mahana

    Manawa

    Nā pōʻai

    65 ℃

    60 sec *e hōʻiliʻili i ka FAM fluorescence

    30

     

    Nā memo

    1.Hiki ke ʻike ʻia ka paʻakai ma lalo o ka paipu paʻa, vortex pōkole a i ʻole e hoʻohuli i nā paipu i nā manawa he nui e hui pono i ka lumi wela.

    2.Hiki ke hoʻonui ʻia ka mahana hopena ma waena o 62 ℃ a me 68 ℃ e like me ke ʻano o nā primers.

    3.Pono e hoʻohālikelike ʻia ka hoʻokolohua, me ka hoʻomākaukau ʻana i ka ʻōnaehana pane, lyophilization, a me ka hoʻoheheʻe ʻana a me ke kaʻina hana hoʻohui;

    4.No ka pale ʻana i ka haumia, pono e hoʻomākaukau i ka ʻōnaehana pane i loko o kahi papa hoʻomaʻemaʻe ultra-maʻemaʻe, ma nā ʻano hoʻohālikelike ʻē aʻe i ka puʻupuʻu fume o ka lumi e pale aku ai i ka hoʻopili pololei ʻana.

     

     

    E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou