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ʻO Taqman Multiplex qPCR Premix HCB5061B Kiʻi Manaʻo
  • Taqman Multiplex qPCR Premix HCB5061B

Taqman Multiplex qPCR Premix


Helu popoki: HCB5061B

Pūʻolo: 100RXN/1000RXN/10000RXN

ʻO Taqman Multiplex qPCR Premix master mix he hanauna hou antibody i hoʻololi ʻia ʻo Taq DNA Polymerase fluroescence quantitative qPCR hiki ke hoʻohana ʻia i ka lyophilization reagent.

Hōʻike huahana

kikoʻī huahana

Helu popoki: HCB5061B

ʻO Taqman Multiplex qPCR Premix master mix he hanauna hou antibody i hoʻololi ʻia ʻo Taq DNA Polymerase fluroescence quantitative qPCR hiki ke hoʻohana ʻia i ka lyophilization reagent.He kūpono ia no ka FAM/HEX/TET/ JOE/ROX etc. ʻO nā ʻimi ʻelua-labelled (pono no nā probes Molecular Beacon).Loaʻa i ka reagent ka hotstart Fast Taq DNA Polymerase, dNTP etc. ʻO ka mea hiki ke hoʻomaloʻo manuahi ma ka mīkini.ʻAʻole i loko o ka huahana nā kala i hoʻoponopono ʻia e ROX a kūpono ia no nā kumu hoʻohālike PCR nui.


  • Mua:
  • Aʻe:

  • Nā ʻāpana

    Lyo-Makaukau TaqMan qPCR Mix

     

    Nā Kūlana Waihona

    Pono e mālama ʻia kēia huahana ma -25 ~ -15 ℃ mai ka mālamalama no 1 makahiki.

     

    Nā kuhikuhi

    1.Pūnaehana pane

    Nā ʻāpana

    Volume (μL)

    Hoʻoholo hope

    Huihui Enzyme

    20

    -

    Puhi Puhi (10μM)

    x

    0.1-0.5μM

    Māmā (10μM)

    x

    50-250nM

    DNA kāpalapala

    1-25

    -

    dd H2O

    a hiki i ka 25

    -

     

    2.Palapala hana pane

    Kaʻina kaʻapuni

    Temp.

    Manawa

    Nā pōʻaiapuni

    Denaturation mua

    95 ℃

    5 min

    1

    Pane hoʻonui

    95 ℃

    15 sec

    45

    Hoʻopili/Hoʻonui

    60 ℃

    30 sec

     Nā memo:Ma nā hihia kūikawā hiki ke hoʻololi ʻia ka mahana annealing/extension e like me ke kumu waiwai Tm, 60 ° C.

     

    Kaʻina Lyophilization

    Kaʻina hana

    Temp.

    Hoʻonohonoho i ka manawa

    Ka lōʻihi manawa  

    Paʻi

    Ma mua o ka hau hau

    -45 ℃

    10 min

    60 min

     

    Hoʻomaloʻo Kumu

    -45 ℃

    10 min

    60 min

    0.01mbar

    -40 ℃

    10 min

    600 min

    0.01mbar

    -35 ℃

    10 min

    120 min

    0.01mbar

    -30 ℃

    20 min

    60 min

    0.01mbar

    -20 ℃

    20 min

    60 min

    0.01mbar

    -10 ℃

    20 min

    60 min

    0.01mbar

    0 ℃

    20 min

    60 min

    0.01mbar

     

    Hoʻomaloʻo ʻelua

    5℃

    10 min

    20 min

    0.01mbar

    0.01mbar

    10 ℃

    10 min

    20 min

    15 ℃

    10 min

    20 min

    0.01mbar

    20 ℃

    10 min

    20 min

    0.01mbar

    25 ℃

    10 min

    240 min

    0.01mbar

     

     Nā memo

    1. No ka noiʻi wale nō kēia huahana.

    2. E ʻoluʻolu e hana me nā ʻaʻahu lab a me nā mīkina lima hoʻopau, no kou palekana.

     
    E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou