Hoʻokahi ʻanuʻu RT-qPCR pahu pahu
wehewehe
ʻO U+ One Step RT-qPCR Probe Kit (Glycerol-free) he glycerol-free one-step RT-qPCR reagent e hoʻohana ana i ka RNA ma ke ʻano he template (e like me RNA virus), i kūpono no ka hoʻomohala ʻana a me ka hoʻolālā ʻana i nā huahana lyophilized.Hoʻohui kēia huahana i ka hana ʻoi loa o ka Reverse Transcriptase i hoʻolaʻa ʻia hoʻokahi a me ka Champagne Taq DNA Polymerase wela-hoʻomaka, me ka hoʻomaloʻo hoʻomaloʻo manuahi maikaʻi maikaʻi, ʻoi aku ka maikaʻi o ka amplification, ke kaulike a me ka kikoʻī, a kūpono me nā ʻano maloʻo maloʻo like ʻole. ʻO nā kaʻina hana. Eia kekahi, hoʻokomo ʻia ka ʻōnaehana anti-pollution dUTP / UDG i loko o ka reagent, hiki ke hana ma ka lumi wela, hoʻopau i ka hopena o ka hoʻohaumia ʻana o ka huahana ma qPCR, a hōʻoia i ka pololei o nā hopena.
Nā Kumu Kumu o RT-qPCR
Hōʻike
| Nā mea ho'āʻo | Hōʻike | Ka hopena |
| (SDS PAGE) Ma'ema'e o ka Enzyme Stock(SDS PAGE) | ≥95% | Holo |
| Endonuclease Activity | ʻAʻole ʻike ʻia | Holo |
| Ka hana Exodulease | ʻAʻole ʻike ʻia | Holo |
| Hanana Rnase | ʻAʻole ʻike ʻia | Holo |
| Ke koena E.coli DNA | ＜1 kope/60 | Holo |
| Pūnaehana ʻIke Hana Hana | 90%≤110% | Holo |
Nā ʻāpana
| Nā ʻāpana | 100rxns | 1,000rns | 5,000 rxns |
| RNase-noa ddH2O | 2*1ml | 20ml | 100ml |
| 5 * hoʻokahi ʻanuʻu hui | 600μl | 6*1ml | 30ml |
| Hoʻokahi ʻanuʻu hui enzyme | 150μl | 2*750μl | 7.5ml |
| 50* Hōʻike ROX Peila 1 | 60μl | 600μl | 3*1ml |
| 50* Hōʻike ROX Peila 2 | 60μl | 600μl | 3*1ml |
a.Hoʻokahi-Step Buffer me ka dNTP Mix a me Mg2+.
b.Aia i loko o ka Enzyme Mix ka hoʻohuli
transcriptase, Hot Start Taq DNA polymerase (hoʻololi antibody) a me ka mea hoʻopaneʻe RNase.
c.Hoʻohana ʻia e hoʻoponopono i ka hewa o nā sinals fluorescene ma waena o nā pūnāwai like ʻole.
c.ROX: Pono ʻoe e koho i ka calibration e like me ke kumu hoʻohālike o ka mea hoʻāʻo.
Nā noi
ʻIke QPCR
Hoʻouna a mālama
Kaʻahele:Puke hau
Kūlana mālama:E mālama i ka -20 ℃.
Shief Life:18 mahina
