Multiplex One Step RT-qPCR Probe Kit (UDG Plus)
wehewehe
ʻO ka Multiplex One Step RT-qPCR Kit (UDG Plus) kahi pahu ʻike wikiwiki RT-qPCR i hoʻokumu ʻia i kūpono no ka PCR hoʻokahi-plex a i ʻole multi-plex quantitative PCR me ka hoʻohana ʻana i ka RNA ma ke ʻano hoʻohālike (e like me ka RNA virus).Hoʻohana ʻia ia e hoʻonui i ka DNA doublestranded mai nā hoʻohālikelike RNA singlestranded e ʻae i ka helu wikiwiki maoli o nā pahuhopu RNA.Ma ka ʻanuʻu transcription hope, hoʻohui ka transcriptase reverse i nā molekole DNA singlestranded (cDNA) i hoʻohui ʻia i ka template RNA.
Kaʻina Hana
Nā ʻāpana
| Nā ʻāpana | 200rxns | 1,000rns | 10,000 rxns |
| RNase-noa ddH2O | 3*1ml | 15ml | 150ml |
| 5 * hoʻokahi ʻanuʻu hui | 800μl | 4*1ml | 40ml |
| Hoʻokahi ʻanuʻu hui enzyme | 200μl | 1ml | 10ml |
| 50* Hōʻike ROX Peila 1 | 80μl | 400μl | 4*1ml |
| 50* Hōʻike ROX Peila 2 | 80μl | 400μl | 4*1ml |
a.Hoʻokahi-Step Buffer me ka dNTP Mix a me Mg2+.
b.Aia ka Enzyme Mix i ka reverse transcriptase, Hot Start Taq DNA polymerase (hoʻololi antibody) a me RNase inhibitor.
c.Hoʻohana ʻia e hoʻoponopono i ka hewa o nā sinals fluorescene ma waena o nā pūnāwai like ʻole.
c.ROX: Pono ʻoe e koho i ka calibration e like me ke kumu hoʻohālike o ka mea hoʻāʻo.
Nā noi
ʻIke i nā pathogens
diagnostics and research of tumor
ʻIke maʻi holoholona
ʻO ka ʻike mua ʻana o nā maʻi hoʻoilina
ʻIke i nā microorganism pathogenic i ka meaʻai
Hoʻouna a mālama
Kaʻahele:Puke hau
Kūlana mālama:E kūʻai ma -30 ~ -15 ℃.
Sheef ola:1 makahiki
