Hoʻokahi ʻanuʻu RT-qPCR pahu pahu

Hoʻolālā kūikawā ʻia ʻo One Step qRT-PCR Probe Kit no qPCR e hoʻohana pololei ana i ka RNA (eg virus RNA) ma ke ʻano he laʻana.Me ka hoʻohana ʻana i nā gene specific primers (GSP), hiki ke hoʻopau i ka transcription reverse a me qPCR i hoʻokahi paipu, e hōʻemi nui ana i nā kaʻina pipetting a me ka pilikia o ka hoʻohaumia ʻana.Hiki ke hoʻopau ʻia i ka 55 ℃ me ka ʻole e pili ana i ka pono a me ka naʻau o qRT-PCR.ʻO ka hoʻohui ʻana i ka hana ʻoi aku ka maikaʻi o HiScript III Reverse Transcriptase a me ka Champagne Taq DNA Polymerase wela wela, me kahi ʻōnaehana buffering optimized, hiki i ka ʻike ʻike o One Step qRT-PCR Probe Kit ke hiki i 0.1 pg o ka huina RNA a i ʻole he 10 mau kope o nā mamana RNA. a ʻoi aku ka ʻoi aku o ka paʻa wela.Hoʻokahi ʻanuʻu qRT-PCR Probe Kit i hāʻawi ʻia ma Master Mix.Aia i loko o ka 5 × One Step Mix kahi buffer optimized a me dNTP/dUTP Mix, a he kūpono ia no nā ʻōnaehana ʻike kikoʻī kiʻekiʻe e pili ana i nā probes lepili fluorescence (e laʻa me TaqMan).