Pākīpika K( wai)
Nā pono
● ʻOi aku ka paʻa a me ka hana enzyme ma muli o nā ʻenehana evolution i kuhikuhi ʻia
● Hoʻomanawanui i ka paʻakai Guanidine
● ʻAʻole RNase, ʻole DNase a me Nickase manuahi, DNA <5 pg/mg
wehewehe
ʻO Proteinase K kahi protease serine kūpaʻa me ka kikoʻī o ka substrate.Hoʻohaʻahaʻa ia i nā protein he nui i ka mokuʻāina ʻoiai i mua o nā detergents.Hōʻike nā hōʻike mai nā haʻawina kristal a me ka molecular structure no ka ʻohana subtilisin me kahi kahua catalytic triad (Asp 39-His 69-Ser 224).ʻO ka pae nui o ka cleavage ʻo ka paʻa peptide e pili ana i ka pūʻulu carboxyl o aliphatic a me nā ʻakika amino ʻala me nā pūʻulu alpha amino.Hoʻohana maʻamau ia no kāna kikoʻī ākea.
ʻO ke kinona
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| ʻAno (Laula) | ʻAʻole waihoʻoluʻu i ka ʻulaʻula |
| ʻAno ( Turbidity) | Mākaʻakaʻa i ka Haʻaleʻa Loa |
| ʻAno | Waiwai |
| Ka hana | ≥800U/ml |
| Paʻakai mg protein / ml | ≥20 mg /ml |
| RNase | ʻAʻohe mea i ʻike ʻia |
| DNase | ʻAʻohe mea i ʻike ʻia |
| Nicakase | ʻAʻohe mea i ʻike ʻia |
Nā noi
ʻO ka pahu diagnostic genetic;
RNA a me DNA pahu pahu;
ʻO ka unuhi ʻana o nā ʻāpana non-protein mai nā ʻiʻo, ka hoʻohaʻahaʻa ʻana i nā haumia protein, e like me
Nā lāʻau lapaʻau DNA a me ka hoʻomākaukau ʻana i ka heparin;
Ka hoʻomākaukau ʻana o ka chromosome DNA e ka pulsed electrophoresis;
Western blot;
Enzymatic glycosylated albumin reagents in vitro diagnostics
Hoʻouna a mālama
Hoʻouna:Ambient
Kūlana mālama:E kūʻai ma -20 ℃ (Loaʻa manawa)/ 2-8 ℃( Pōkole)
Manaʻo ʻia ka lā hoʻāʻo hou:2 Makahiki
