ʻO Proteinase K (Lyophiled Powder)
Nā pono
● ʻOi aku ka paʻa a me ka hana enzyme ma muli o nā ʻenehana evolution i kuhikuhi ʻia
● Hoʻomanawanui i ka paʻakai Guanidine
● ʻAʻole RNase, DNase manuahi a me Nickase manuahi, DNA <5 pg/mg
wehewehe
ʻO Proteinase K kahi protease serine kūpaʻa me ka kikoʻī o ka substrate.Hoʻohaʻahaʻa ia i nā protein he nui i ka mokuʻāina ʻoiai i mua o nā detergents.Hōʻike nā hōʻike mai nā haʻawina kristal a me ka molecular structure no ka ʻohana subtilisin me kahi kahua catalytic triad (Asp 39-His 69-Ser 224).ʻO ka pae nui o ka cleavage ʻo ka paʻa peptide e pili ana i ka pūʻulu carboxyl o aliphatic a me nā ʻakika amino ʻala me nā pūʻulu alpha amino.Hoʻohana maʻamau ia no kāna kikoʻī ākea.
ʻO ke kinona
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| wehewehe | ʻO ka pauka amorphous keʻokeʻo a keʻokeʻo, Lyophiled |
| Ka hana | ≥30U/mg |
| Solubility (50mg Powder/mL) | Akaka |
| RNase | ʻAʻohe mea i ʻike ʻia |
| DNase | ʻAʻohe mea i ʻike ʻia |
| Nickase | ʻAʻohe mea i ʻike ʻia |
Nā noi
ʻO ka pahu diagnostic genetic;
RNA a me DNA pahu pahu;
ʻO ka unuhi ʻana o nā ʻāpana non-protein mai nā ʻiʻo, ka hoʻohaʻahaʻa ʻana i nā haumia protein, e like me
Nā lāʻau lapaʻau DNA a me ka hoʻomākaukau ʻana i ka heparin;
Ka hoʻomākaukau ʻana o ka chromosome DNA e ka pulsed electrophoresis;
Western blot;
Enzymatic glycosylated albumin reagents in vitro diagnostics
Hoʻouna a mālama
Hoʻouna:Ambient
Kūlana mālama:E kūʻai ma -20 ℃ (Loaʻa manawa)/ 2-8 ℃( Pōkole)
Manaʻo ʻia ka lā hoʻāʻo hou:2 Makahiki
Hoʻomalu
E hoʻohana i nā mīkina lima pale a me nā maka aniani i ka wā e hoʻohana ai a i ke kaupaona ʻana, a mālama pono i ka ea ma hope o ka hoʻohana ʻana.Hiki i kēia huahana ke hoʻoulu i ka maʻi ʻili.Hoʻopilikia i ka maka.Inā inhaled, hiki ke hoʻoulu i ka maʻi a me nā hōʻailona hānō a i ʻole dyspnea.Hiki ke hoʻopilikia i ka hanu.
Wehewehe Unite
Hoʻokahi ʻāpana (U) i wehewehe ʻia e like me ka nui o ka enzyme e koi ʻia e hydrolyze casein e hana ai i ka 1 μmol tyrosine i kēlā me kēia minuke ma lalo o kēia mau kūlana.
