dATP/ dCTP/ dGTP/ dTTP/ dUTP (100mM )
wehewehe
ʻO kēia huahana he wai kala ʻole.He kūpono ia no nā ʻano hoʻokolohua molecular biology maʻamau e like me ka PCR amplification, PCR manawa maoli, cDNA a i ʻole synthesis DNA maʻamau, DNA sequencing a me ka lepili.Hiki ke hoʻoheheʻe ʻia me ka wai ultra-maʻemaʻe, a hoʻoponopono ʻia i ka pH 7.0 me ka hoʻonā NaOH maʻemaʻe kiʻekiʻe, me ka maʻemaʻe ≥ 99% (HPLC).Ma hope o ka ʻike ʻana, ʻaʻohe ona DNase, RNase a me phosphotase.Hiki ke hoʻohana pololei ʻia i loko o nā ʻano like ʻole o ka molecular biological reactions e like me PCR.
ʻO ke kinona
Ka wehewehe
| Nā mea ho'āʻo | Nā kikoʻī |
| Ka nana aku | Haʻina kala ʻole |
| Hoʻopaʻa | 100mM ±3% |
| Maʻemaʻe (HPLC) | ≥99% |
| PH(22-25°C) | 7.0±0.1 |
| Ka Mana Mana1. ʻAʻole kēia hoʻomākaukau ʻana i ka DNase a me RNase contamination 2. Ua hoʻāʻo ʻia kēia ʻāpana o ka dNTP me Taq DNA polymerase a me Pfu DNA polymerase Ua ho'āʻo ʻia kēia ʻāpana o ka NTP me Q-PCR | |
Nā noi
PCR lōʻihi (20 kb)
cDNA synthesis a me RT-PCR
PCR manawa maoli
PCR maʻamau
PCR kūpaʻa kiʻekiʻe
Hoʻouna a mālama
Kaʻahele:Puke hau
Kūlana mālama:E kūʻai ma -25～-15 ℃
Manaʻo ʻia ka lā hoʻāʻo hou:2 makahiki
Nā memo
1) Hiki ke hoʻoheheʻe ʻia ma ka lumi wela.Ma hope o ka hemo ʻana, pono e mālama ʻia i loko o kahi pahu hau a i ʻole ʻauʻau hau.Ma hope o ka hoʻohana ʻana, pono e mālama ʻia ma -25～-15 ℃ koke.
2) No kou palekana a me kou olakino, e ʻoluʻolu e ʻaʻahu i nā ʻaʻahu lab a me nā mīkina lima hoʻopau no ka hana.
