Ribonuclease A (RNase A)

ʻO Ribonuclease A (RNase A) kahi polypeptide i hoʻopaʻa ʻia me 4 mau paʻa disulfide me ke kaumaha molekala ma kahi o 13.7 kDa.ʻO RNase A kahi endoribonuclease e hoʻohaʻahaʻa pono i ka RNA kaula hoʻokahi ma nā koena C a me U.Ma keʻano kikoʻī, ʻike ka cleavage i ka paʻa phosphodiester i hoʻokumu ʻia e ka 5'-ribose o kahi nucleotide a me ka pūʻulu phosphate ma ka 3'-ribose o ka nucleotide pyrimidine e pili ana, no laila ua hydrolyzed ka 2', 3' - Cyclic phosphates i ka like. 3'-nucleoside phosphates (e like me ka pG-pG-pC-pA-pG i hoʻokaʻawale ʻia e RNase A e hoʻohua i ka pG-pG-pCp a me A-PG).ʻO RNase A ka mea ikaika loa i ka hoʻokaʻawale ʻana i ka RNA kaula hoʻokahi a ʻeleʻele i nā ʻano hopena like ʻole: ma ka haʻahaʻa haʻahaʻa o ka paʻakai (0 a 100 mM NaCl), hiki ke hoʻohana ʻia no ka hoʻokaʻawale ʻana i ka RNA kaula hoʻokahi, RNA pālua ʻelua, a me nā kaula RNA i nā huila RNA-DNA.ʻoiai ma ka paʻakai kiʻekiʻe (≥0.3 M), hiki iā RNase A ke hoʻokaʻawale pono i ka RNA kaula hoʻokahi.