Bst 2.0 DNA Polymerase(Glycerol free)
Loaʻa ka Bst DNA polymerase V2 mai Bacillus stearothermophilus DNA Polymerase I, nona ka hana 5′→3′ DNA polymerase a me ka hana hoʻololi kaulahao ikaika, akā ʻaʻohe 5′→3′ exonuclease hana.He kūpono loa ka Bst DNA Polymerase V2 no ka hoʻoneʻe ʻana i ke kaula, ka LAMP hoʻonui isothermal (Loop mediated isothermal amplification) a me ke kaʻina wikiwiki.
Nā ʻāpana
| ʻāpana | HC5005A-01 | HC5005A-02 | HC5005A-03 |
| BstDNApolymerase V2(Glycerol-free)(8U/μL) | 0.2 mL | 1 mL | 10 mL |
| 10×HC Bst V2 Hoʻopaʻa | 1.5 mL | 2×1.5 mL | 3×10 mL |
| MgSO4(100mM) | 1.5 mL | 2×1.5 mL | 2×10 mL |
Nā noi
1.LAMP isothermal amplification
2.DNA strand hoʻoneʻe hoʻokahi pane
3.High GC gene sequencing
4.DNA sequencing o nanogram pae.
Kūlana Waihona
ʻO ka halihali ma lalo o 0°C a mālama ʻia ma -25°C~-15°C.
Wehewehe Unite
Hoʻokahi ʻāpana i wehewehe ʻia ʻo ka nui o ka enzyme e hoʻokomo i 25 nmol o dNTP i loko o ka mea hiki ke hoʻoheheʻe ʻia i loko o 30 mau minuke ma 65°C.
Ka Mana Mana
1.Hoʻomaʻemaʻe Protein (SDS-PAGE):ʻO ka maʻemaʻe o Bst DNA polymerase V2 he ≥99% i hoʻoholo ʻia e ka SDS-PAGE analysis me ka hoʻohana ʻana i ka Coomassie Blue detection.
2.Ka hana Exonuclease:ʻO ka incubation o ka hopena 50 μL i loaʻa ka liʻiliʻi o 8 U o Bst DNA polymerase V2 me 1 μg λ -Hind Ⅲ digest DNA no 16 mau hola ma 37 ℃ hopena ʻaʻohe degradable ʻike ʻia e like me ka mea i hoʻoholo ʻia.
3.Hana Nickase:ʻO ka hoʻoulu ʻana o kahi hopena 50 μL i loaʻa ka liʻiliʻi o 8 U o Bst DNA polymerase V2 me 1 μg pBR322 DNA no 16 mau hola ma 37°C ʻaʻohe hopena i ʻike ʻia e like me ka mea i hoʻoholo ʻia.
4.Hana Hana:Incubation o ka 50 μL hopena i loaʻa ka liʻiliʻi o 8 U o Bst DNA polymerase V2 me 1.6 μg MS2 RNA no 16 mau hola ma 37°C ka hopena ʻaʻole i ʻike ʻia ka degradation e like me ka mea i hoʻoholo ʻia.
5.E. coli DNA:ʻO 120 U o Bst DNA polymerase V2 ka nānā ʻia no ka loaʻa ʻana o E. coli genomic DNA me ka hoʻohana ʻana i TaqMan qPCR me nā primers kikoʻī no ka E. coli 16S rRNA locus.ʻO ka E. coli genomic DNA contamination he ≤1 Kope.
LAMP Pane
| Nā ʻāpana | 25μL |
| 10×HC Bst V2 Hoʻopaʻa | 2.5 μL |
| MgSO4 (100mM) | 1.5 μL |
| dNTP (10mM kēlā me kēia) | 3.5 μL |
| SYTO™ 16 ʻōmaʻomaʻo (25×)a | 1.0 μL |
| Huina muab | 6 μL |
| Bst DNA Polymerase V2 (Glycerol-free) (8 U/uL) | 1 μL |
| Papahana | × μL |
| ddH₂O | A hiki i ka 25 μL |
Nā memo:
1) a.SYTOTM 16 ʻOmaomao (25×): E like me nā pono hoʻokolohua, hiki ke hoʻohana ʻia nā mea pena ʻē aʻe i pani;
2) b.Hui mua: loaʻa ma ka hui ʻana i 20 µ M FIP, 20 µ M BIP, 2.5 µ M F3, 2.5 µ M B3, 5 µ M LF, 5 µ M LB a me nā puke ʻē aʻe.
Pane a me ke kūlana
1 × HC Bst V2 Buffer, aia ka mahana incubation ma waena o 60°C a me 65°C.
ʻAʻohe wela
80 °C, 20 mau minuke
