RT-LAMP Fluorescent (Lyophilized poepoe)
Loaʻa i ka RT-LAMP Fluorescent Master Mix (Lyophilized Powder) ka hoʻopaʻa ʻana i ka hopena, Bst DNA polymerase a me ka transcriptase hoʻohuli i ka wela, ka mea pale lyophilized, a me ka wai kala.Aia ka huahana ma ke ʻano o nā lyophilized beads, a pono e hoʻohui ʻia nā primers a me nā templates no ka hoʻohana.
Mea Hoʻohui
RT-LAMP Fluorescent Master Mix (Lyophilized Powder)
Hoʻohana
No ka DNA a i ʻole RNA isothermal amplification
Nā Kūlana Waihona
ʻO ka lawe ʻana a mālama ʻia ma 2 ~ 8 ° C.
Noho ʻia: 12 mahina.
Kūkākūkā
1. E lawe i ka helu kūpono o nā lyophilized beads e like me ka helu o nā hoʻokolohua.
2. Hoʻomākaukau ʻōnaehana pane
| ʻāpana | ʻAiʻai |
| RT-LAMP Fluorescent Master Mix (Lyophilized Powder) | 2 lei |
| kala ʻulaʻula | x μL |
| 10 × Hui Pūmua a | 5 μL |
| Palapala DNA/RNA b | n μL |
| Huina | 50 μL |
Nā memo:
1) a.10×Primer Mix : 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;
2) b.Manaʻo ʻia e hoʻoheheʻe ʻia ka ʻakika nucleic i ka wai DEPC.
Hyasen Biotechnology Co., Ltd
3) Papahana pane
| Mahana | Manawa | Nā pōʻaiapuni |
| 65 ℃ | 60s a | 30 |
Nā memo:
1. E hōʻiliʻili i ka hōʻailona fluorescent
Nā memo
1. Hiki ke hoʻonui ʻia ka mahana hopena ma waena o 62°C a me 68°C e like me ka primernā kūlana;
2. Pono ka hoʻokolohua e hoʻohālikelike i ka hana, me ka hoʻomākaukau ʻana i ka ʻōnaehana pane.ka hana laʻana a me ka dosing;
3. No ka pale ʻana i ka contamination, ʻōlelo ʻia e hoʻomākaukau i ka ʻōnaehana hopena i kahi papaʻaina ultra-cleana hoʻohui i nā mamana i ka fume hood ma nā keʻena ʻē aʻe e pale aku i ka hoʻopiʻi hoʻopunipuni.
