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BsaI HCP1014A Kiʻi Manaʻo
  • BsaI HCP1014A

BsaI


Helu popoki:HCP1014A

Pūʻolo: 50μL/250μL/1mL/10mL/100mL

BsaI, kahi IIs restriction endonuclease restriction endonuclease, i loaʻa mai ka arecombinant E.

Hōʻike huahana

ʻikepili huahana

ʻO BsaI, kahi IIs restriction endonuclease restriction endonuclease, ua loaʻa mai ka arecombinant E. coli strain e lawe ana i ka gene BsaI cloned a hoʻololi ʻia mai Bacillus stearothermophilus. He ʻano kikoʻī like me ka enzyme maoli a me ka hana hōʻemi hoʻi.ʻO kāna kaʻina ʻike a me nā wahi ʻokiʻoki penei:

5' GGTCTC(N)

3'······CCAGAG(NNNNN)···5'


  • Mua:
  • Aʻe:

  • Nā ʻāpana

    BsaI(20U/μL), 10xBsaI Hoʻopaʻa

     

    Waihona

    E kūʻai ma -25 ℃ ~ -15 ℃, kūpono no 1 makahiki (E hōʻalo i nā pōʻai hoʻoheheʻe hauʻoli).

     

    Paʻa mālama

    10mM Tris-HCl, 200mM NaCl, 1mM DTT 0.1mM EDTA, 200µg/ml Recombinant Albumin, 50% Glycerol.(pH 7.4 @ 25°C).

     

    Nā hiʻohiʻona huahana

    ʻO ka hana kiʻekiʻe, ka ʻai wikiwiki;

    1. Haʻahaʻa hoku hana, e hōʻoia 'ia oki pololei e like me "scalpel";

    2. Me ka loaʻa ʻole o ka BSA a me ke kumu holoholona.

    Methylation Sensitivity

    Dam methylation: ʻAʻole paʻakikī;

    Dcm methylation: Hoʻopilikia ʻia e kekahi mau hui ʻana o ka overlapping;

    CpG Methylation: Paʻa ʻia e kekahi mau hui o ka overlapping.

     

     

    Wehewehe Unite

    Hoʻokahi pūʻulu e wehewehe i ka nui o ka enzyme e pono ai e hoʻoheheʻe i 1 µg o Internal Control DNA i 1 hola ma 37°C i ka nui o ka nui o ka hopena o 50 µL.

     

    Ka Mana Mana

    Hoʻomaʻemaʻe Protein (SDS-PAGE): ʻO ka maʻemaʻe o Bsa I ≥ 95% i hoʻoholo ʻia e ka SDS-PAGE analysis me ka hoʻohana ʻana i ka Coomassie Blue detection.

    RNase:20 U o Bsa I me 1.6 μg MS2 RNA no 16hoursat37 ℃ hua ʻole i ka hoʻohaʻahaʻa e like me ka hoʻoholo ʻana e ka electrophoresis gel agarose.

    Nā Hana DNase Kūikaʻi ʻole:20 Uof BsaI me 1 μg PhiX174 DNA no 16 mau hola ma 37 ℃ ʻaʻole e hāʻawi i ka DNA keu aku e like me ka hoʻoholo ʻana e ka electrophoresis gel agarose.

    Ligation a me ka ʻoki ʻana:Ma hope o ka ʻai ʻana o 1 μg

    E.coli DNA:2Uof VacciniavirusCapping Enzyme ua hoʻopaʻa ʻia no ka loaʻa ʻana o E. coli genomic DNA me ka hoʻohana ʻana i TaqManqPCR me nā primers kikoʻī no ka E. coli 16S rRNA locus.ʻO ka E. coli genomic DNA contamination ʻo ≤1 E. coli genome.

    Endotoxin bacterial:LAL-ho'āʻo, e like me ka ChinesePharmacopoeiaIV2020edition, gel kaʻina hoʻāʻo palena, kānāwai maʻamau (1143).Pono ka maʻiʻo endotoxin bacteria ≤10 EU/mg.

     

    Pūnaehana pane a me nā kūlana

    ʻāpana

    Volume

    BsaI(20 U/μL)

    1μL

    DNA

    1μg

    10 x BsaI Hoopaa

    5μL

    dd H2O

    A hiki i 50 μL

    Incubate ma 37 ° C no 15-30 mau minuke.Hoʻopau wela: 80°C no 20 min.

    Hiki i ka ʻōnaehana hopena a me nā kūlana i ʻōlelo ʻia ke hāʻawi i ka hopena digestion enzyme maikaʻi loa, ʻo ia no ke kuhikuhi wale nō, e ʻoluʻolu e hōʻike i nā hopena hoʻokolohua no nā kikoʻī.

     

    Nā noi

    Kaohi ʻana i ka Enzyme DigestionFast Cloning.

     

    Nā memo no ka hoʻohana ʻana

    1. ʻO ka nui o ka enzyme ≤ 1 / 10 o ka nui o ka hopena.

    2. Staractivitymayoccurwhenglycerol ʻoi aku ka nui ma mua o 5%.

    3. Hiki ke hana ʻia ka ʻokiʻoki ke loaʻa ka substrate ma lalo o ka ratio i ʻōlelo ʻia.

     

    E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou