Endonuclease ka palena o BsaI

Maʻemaʻe: 20U / μL

Pūʻolo: 50μL, 250μL, 1ml,10ml, 100ml

Huahana Huahana

wehewehe

ʻO ka enzyme restriction BsaI he ʻano II restriction endonuclease i loaʻa mai ka protein recombinant i hoʻopaʻa ʻia e ka BspQI gene ma Bacillus sphaericus i hōʻike ʻia e E.coli.Hoʻohana nui ʻia nā endonucleases hoʻopaʻa ʻia i ka palapala ʻāina gene, cloning a me nā māla ʻē aʻe.ʻOki wikiwiki kēia enzyme i ka DNA no ka linearization gene efficient Bsa I , he IIs restriction endonuclease restriction endonuclease, i loaʻa mai i ka recombinant E. coli strain e lawe ana i ka gene BsaI cloned a hoʻololi ʻia mai Bacillus stearothermophilus.i like like me nā enzymes maoli a me ka hoʻemi ʻana i ka hana hōkū.ʻO kāna kaʻina ʻike a me nā wahi ʻokiʻoki penei:

5'······GGTCTC (N)

3'······CCAGAG(NNNNN)······5'

Hoʻolālā Kimia

Nā hiʻohiʻona huahana

• Loaʻa i ka endonuclease palena ka hana enzymatic kiʻekiʻe a hoʻopau koke i ka hopena digestion.

• Haʻahaʻa haʻahaʻa non-specific endonuclease hana e hōʻoia pololei "scalpel" oki.

• ʻAʻohe ʻōnaehana BSA, e hōʻemi ana i ka pollution kumu wela.

Hōʻike

Nā mea ho'āʻo	Nā kikoʻī
Hana Enzyme	20 U/µL
Maʻemaʻe (SDS-PAGE)	≥95%
Rnase	ʻAʻohe mea i ʻike ʻia
Hana DNase Kūikaʻi ʻole	ʻAʻole ʻike ʻia
Hana Hoku	ʻAʻole ʻike ʻia
Ligation a Recutting	Ma hope o ka ʻai ʻana, hiki ke hoʻopaʻa ʻia nā ʻāpana DNA.A hiki ke ʻoki ʻia kēia mau ʻāpana ligated me Bsal.
Endotoxins(LAL-hoʻāʻo)	≤10 EU/mg
E.Coli DNA	≤1 Kope/2U

Ka hoomalu maikai

Maʻemaʻe ≥ 95%, ʻAʻohe DNase, RNase hana, Hoʻokipa DNA koena ≤ 100pg/mg, koena protein host ≤ -50ppm, koena endotoxin ≤ 10EU/mg, ʻAʻohe hana protease, sterile, ʻaʻohe mycoplasma

Palapala noi

E ʻeli i ka plasmid e hoʻomākaukau i kahi ʻāpana DNA linearized ma ka hopena o Poly (A/T/C/G);

Linearize plasmid template ma mua o ka transcription in vitro;

Kaohi ʻana;

Wehewehe Unite

Hoʻokahi ʻāpana i wehewehe ʻia ʻo ka nui o ka enzyme e koi ʻia e hoʻokaʻawale i 1 µg o ka DNA Mana kūloko i loko o 1 hola ma 37°C i ka nui o ka hopena o 50 µL.