T4 RNA ligase
wehewehe
ʻO kēia huahana he ATP-dependent T4 RNA ligase (T4 RNA Ligase I) i hōʻike hou ʻia e E. coli.Hoʻokumu kēia enzyme i ka hoʻokumu ʻana i nā mea paʻa phosphodiester ma waena o nā oligonucleotides, RNA i kau ʻia hoʻokahi a me DNA intermolecular/ intra-molecular 5'-PO4 a me 3'-OH.
Wehewehe Unite
Hoʻokahi ʻāpana i wehewehe ʻia ʻo ka nui o ka enzyme e pono ai e hoʻohuli i 1 nanomole o 5'-[³²P] rA16 i loko o kahi ʻano pale-phosphatase i loko o 30 mau minuke ma 37°C.
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| Hana Enzyme | 10 U/μL |
| Haʻina Protein | ≥ 95% |
| Nickase | ʻAʻole ʻike ʻia |
| Hana Hana | ʻAʻole ʻike ʻia |
| Ecoli gDNA | ≤1 kope/50U |
| Endotoxin | LCL-Ho'āʻo,≤10EU/mg |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:Hau maloo
Waihona:E kūʻai ma -25~-15°C (hōʻalo i ka maloʻo a me ka hoʻoheheʻe pinepine ʻana)
Manaʻo ʻia e hoʻāʻo hou i ke ola:2 makahiki
huahana pili
E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou
