ʻO ka BspQI Restriction Endonuclease
wehewehe
Hiki ke hōʻike hou ʻia ka BspQI ma E. coli hiki ke ʻike i nā wahi kikoʻī a hana ʻia ma lalo o nā kūlana GMP.Hoʻohana nui ʻia ia i ka palapala gene, cloning a me nā māla ʻē aʻe.ʻOki wikiwiki kēia enzyme i ka DNA no ka linearization gene efficient.
5'······GGTCTC(N)1·····3'
3'······CCAGAG(N)4·····5'
Hoʻolālā Kimia
Wehewehe Unite
Hoʻokahi ʻāpana i wehewehe ʻia e like me ka nui o ka enzyme e pono ai e hoʻoheheʻe i 1 µg o λ DNA i 1 hola ma 50°C i ka nui o ka hopena o 50 µl.
Nā hiʻohiʻona huahana
• Loaʻa i ka endonuclease palena ka hana enzymatic kiʻekiʻe a hoʻopau koke i ka digestion.
• Haʻahaʻa haʻahaʻa non-specific endonuclease hana e hōʻoia pololei "scalpel" oki.
• ʻAʻohe ʻōnaehana BSA, e hōʻemi ana i ka pollution kumu wela.
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| Māʻemaʻe Protein | ＞98% |
| Ke koena o ka Protease | ʻAʻohe mea i ʻike ʻia |
| Hoʻohui a ʻoki ʻoki | ʻO ka hoʻoemi ʻana i ka hana maʻamau> 95% |
| DNase | ʻAʻohe mea i ʻike ʻia |
| Nicaknase | ʻAʻohe mea i ʻike ʻia |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:Hoʻouna ʻia ma lalo o 0 °C
Waihona:E mālama i ka -20 °C
Manaʻo ʻia e hoʻāʻo hou i ke ola:2 makahiki
