ʻO ka pahu hōʻike DNase (Fluorescence)
wehewehe
Hoʻokumu ʻia ka pahu ʻike DNase ma kahi hōʻike DNA i kapa ʻia i ka fluorophore.Ke loaʻa ʻole ka hana DNase i ka hāpana, paʻa ka probe a ʻaʻole hoʻopuka i kahi hōʻailona fluorescent;ke loaʻa ka hana DNase i ka hāpana, hoʻohaʻahaʻa ʻia ka ʻimi noiʻi, e hopena i kahi hōʻailona fluorescence i hoʻonui ʻia;ʻO ka nui o ka piʻi ʻana o ka hōʻailona fluorescence e pili pono me ka helu a me ka hana o nā enzymes.E hoʻohana i ka mea heluhelu microplate fluorescence e ana i ka nalu nalu o ex/em=485/525nm e hoʻoholo ai inā ua haumia ka hāpana e DNase
Hoʻolālā Kimia
haku mele
inoa | 48 Hoao | 192Hoao |
10× hoʻonā hopena | 0.5mL | 2.0mL |
ʻimi DNA | 1 paipu | 1 paipu |
TE pale | 0.5mL | 2.0mL |
DNase I maʻamau (2U/μL) | 10μL | 20μL |
Paʻa hoʻoheheʻe maʻamau | 6mL | 12mL |
DNase&RNase wai ʻole | 25mL | 25mL |
DNase RNase hele aku | 50mL | 50mL |
Hōʻike
Nā mea ho'āʻo | Nā kikoʻī |
Ka nana aku | Hoʻopiha piha ʻia a ʻaʻohe kahe wai |
palena ʻike | DNase I: 1.25x10-6U/μL |
pololei | ʻO ka hoʻāʻo Intra CV ≤ 10% |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:Hau maloo
Waihona:E kūʻai ma -25~-15°C (hōʻalo i ka maloʻo a me ka hoʻoheheʻe pinepine ʻana)
Manaʻo ʻia e hoʻāʻo hou i ke ola:1 makahiki
huahana pili
E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou