AMPPD-Kemiluminescent substrate
wehewehe
He ʻelua ʻāpana koʻikoʻi ka AMPPD i kāna ʻano molekala.ʻO kekahi ka dioxygen
ʻO ke apo tetrad e hoʻopili ai i ke apo benzene a me ka adamantane, hiki ke wāwahi a hoʻokuʻu i nā kiʻi.ʻO ka mea ʻē aʻe ka hiki ʻana o ka alkaline phosphatase, kahi e hoʻopau ai i ka wehe ʻana o nā pūʻulu phosphate a hana i kahi waena paʻa ʻole.ʻO ka intermediate a laila spontaneously decomposed a emitted photons, a me ka helu o nā kiʻi i hoʻokuʻu ʻia ua like ia me ka manaʻo o ALP i loko o ka hopena, i kūpono no ka ʻōnaehana immunoassay chemiluminescence me ALP e like me ka enzyme i kapa ʻia.
E hoʻohana:
ʻO ka AMPPD kahi wai chemiluminescent me ka ʻike kiʻekiʻe, kūpaʻa a me ka ratio signal-to-noise, hiki ke hoʻohana ʻia no ka immunodetection o Alkaline Phosphatase (ALP) ma ke ʻano he māka.
Hoʻolālā Kimia
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| wehewehe | He wai ʻōmaʻomaʻo melemele |
| Paʻa | Ua mālama ʻia ka waiwai Iuminescence o 37 ℃ ma luna o 90% ma hope o 7 mau lā o ka incubation ma lalo o ka pale māmā. |
| waiwai pH | 9.5±0.05 |
| Ke kumu waiwai | 200-10000 |
| ʻO ka waiwai luminecence kiʻekiʻe kiʻekiʻe CV waiwai (i hoʻohālikelike ʻia me ka maʻamau, ʻo 150+5μL nā kūlana hopena, a ʻo 0.0032U/mL ka nui o ka enzyme). | ≤10% |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:Ambient
Waihona :E mālama i ka 2-8 ° C, pale ʻia mai ka mālamalama
Manaʻo ʻia e hoʻāʻo hou i ke ola:2 makahiki
