D-Lactate dehydrogenase(LDH)
wehewehe
Hoʻohana ʻia ka enzyme no ka wehe ʻana i ka pyruvate i nā hoʻoholo e hana pū ana me NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Hoʻohana
1. ʻO ka phosphatase alkaline i hui pū ʻia me nā proteins (antibodies, streptavidin etc.) hiki ke hoʻomaopopo pono i nā molekele i hoʻopaʻa ʻia, a hiki ke hoʻohana ʻia i ka ELISA, WB a me ka ʻike histochemical;
2. Hiki ke hoʻohana ʻia ka phosphatase alkaline e dephosphorize i ka 5 '-terminal o DNA a i ʻole RNA e pale aku i ka pilina pilikino;
3. Hiki ke hoʻopaʻa inoa ʻia ka DNA dephosphorylated a i ʻole RNA e ka radio-labeled phosphates (ma o T4 poly-nucleotide kinase)
Hoʻolālā Kimia
ʻO ka hana hoʻoheheʻe
D-Lactate + NAD+→ Pyruvate +NADH + H+
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| wehewehe | ʻO ka pauka amorphous keʻokeʻo, lyophilized |
| Ka hana | ≥200U/mg |
| Maʻemaʻe (SDS-PAGE) | ≥90% |
| Solubility (10mg pauda/mL) | Akaka |
| NADH/NADPH oxidase | ≤0.01% |
| Malate dehydrogenase | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| Pyruvate kinase | ≤0.03% |
| ʻO Glutamate Dehydrogenase | ≤0.003% |
| Aspartate aminotransferase | ≤0.001% |
| Alamine aminotransferase | ≤0.001% |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:AmbIent
Waihona :E kūʻai ma -20°C(Loaʻa), 2-8°C (pōkole)
Ho'āʻo hou ʻiaola:2 makahiki
