Phospatase Alkalina (ALP)
wehewehe
Loaʻa ʻia ka Alkaline Phosphatase mai kahi recombinant E. coli strain e lawe ana i ka gene TAB5.Hoʻopili ka enzyme i ka dephosphorylation o 5' a me 3' hopena o DNA a me RNA phosphomonoesters.Eia kekahi, hoʻoheheʻe ia i ka ribose, a me ka deoxyribonucleoside triphosphates (NTPs a me dNTPs).Hana ʻo TAB5 Alkaline Phosphatase ma nā 5' e puka ana, 5' i hope a me nā welau pōpō.Hiki ke hoʻohana ʻia ka Phosphatase i nā noi biology molecular, e like me ka cloning a i ʻole ka hoʻopaʻa inoa ʻana i ka hopena no ka wehe ʻana i nā hopena phosphorylated o DNA a i ʻole RNA.I nā hoʻokolohua cloning, pale ka dephosphorylation i ka linearized plasmid DNA mai ka ligation ponoʻī.Hiki iā ia ke hoʻohaʻahaʻa i nā dNTP i hoʻohui ʻole ʻia i nā hopena PCR no ka hoʻomākaukau ʻana i kahi mamana no ke kaʻina hana DNA.ʻAʻole hiki ke hoʻopau ʻia ka enzyme ma ka hoʻomehana ʻana ma 70 ° C no 5 mau minuke, a laila e hoʻoneʻe ʻia ka phosphatase ma mua o ka hoʻopaʻa ʻana a i ʻole ka hoʻopau ʻana i ka lepili ʻana.
Hoʻohana
1. ʻO ka phosphatase alkaline i hui pū ʻia me nā proteins (antibodies, streptavidin etc.) hiki ke hoʻomaopopo pono i nā molekele i hoʻopaʻa ʻia, a hiki ke hoʻohana ʻia i ka ELISA, WB a me ka ʻike histochemical;
2. Hiki ke hoʻohana ʻia ka phosphatase alkaline e dephosphorize i ka 5 '-terminal o DNA a i ʻole RNA e pale aku i ka pilina pilikino;
3. Hiki ke hoʻopaʻa inoa ʻia ka DNA dephosphorylated a i ʻole RNA i luna e nā radio-labeled phosphates (ma o T4 poly-nucleotide kinase)
Hoʻolālā Kimia
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| Hana Enzyme | 5U/μL |
| Endonuclease Activity | ʻAʻole ʻike ʻia |
| Ka hana Exonuuclease | ʻAʻole ʻike ʻia |
| Haʻawina Nicking | ʻAʻole ʻike ʻia |
| Hana Hana | ʻAʻole ʻike ʻia |
| E.coli DNA | ≤1 kope/5U |
| Endotoxin | LAL-Ho'āʻo, ≤10EU/mg |
| Maemae | ≥95% |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:AmbIent
Waihona :E mālama i ka 2-8°C
Manaʻo ʻia e hoʻāʻo houola:2 makahiki
