Hexokinase (HK)
wehewehe
E hoʻohana i ka Hexokinase no ka hoʻoholo ʻana i ka D-glucose, D-fructose, a me D-sorbitol i ka meaʻai a i ʻole nā laʻana noiʻi olaola.Hoʻohana pū ʻia ka enzyme no ka hoʻāʻo ʻana i nā saccharides ʻē aʻe i hoʻololi ʻia i ka glucose a i ʻole ka fructose, a no laila pono ia i ka hoʻāʻo ʻana i nā glycosides he nui.
Inā hoʻohana ʻia ʻo Hexokinase me ka glucose-6-phosphate dehydrogenase (G6P-DH)* (hōʻike i ka glucose6-phosphate i hoʻokumu ʻia e Hexokinase), ʻaʻole pono nā laʻana o ka phosphate kiʻekiʻe e like me ka G6P-DH i hoʻopaʻapaʻa ʻia e ka phosphate.
Hoʻolālā Kimia
Kumu Manaʻo
D-Hexose + ATP --Mg2+→ D-Hexose-6-phosphate + ADP
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| wehewehe | Keʻokeʻo a liʻiliʻi melemele pauda amorphous, lyophilized |
| Ka hana | ≥30U/mg |
| Maʻemaʻe (SDS-PAGE) | ≥90% |
| Solubility (10mg pauka/ml) | Akaka |
| Nā mea pale | ≤0.01% |
| ATPase | ≤0.03% |
| Phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| Glucose-6-Phosphate dehydrogenase | ≤0.01% |
| NADH/NADPH oxidase | ≤0.01% |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele: Ambient
Waihona :E kūʻai ma -20°C(Loaʻa), 2-8°C (pōkole)
Ho'āʻo hou ʻiaola:1 makahiki
huahana pili
E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou
