Glucose Dehydrogenase(GDH)

ʻO Glutamate Dehydrogenase (GDH) kahi enzyme mitochondrial e hoʻopaʻa i ka deamination oxidative reversible o ka glutamate i a-ketoglutarate a lawelawe ma ke ʻano he loulou nui ma waena o nā ala anabolic a me catabolic.I loko o nā mammals, pili ka GDH i ka hoʻoponopono allosteric a he hana kiʻekiʻe kona i loko o ke ake, ka puʻupaʻa, ka lolo, a me ka pancreas.Hiki ke hoʻohana ʻia ka hana GDH i loko o ka serum e hoʻokaʻawale i waena o nā maʻi ate ma muli o ka mumū o ke ake, ʻaʻole e hōʻike i ka hana GDH serum kiʻekiʻe, a me nā maʻi e hopena i ka necrosis hepatocyte, e hopena i ka serum GDH kiʻekiʻe.

Hoʻoholo ʻia ka hana GDH e ka hoʻāʻo ʻana i ka enzyme i hoʻopau ʻia ka glutamate e ka GDH e hoʻohua ana i ka NADH, ka mea e pane me ka probe e hana ana i kahi huahana colorimetric (450 nm) e like me ka hana GDH i kēia manawa.Hoʻokahi ʻāpana o GDH ka nui o ka enzyme e hoʻohua ai i 1.0 mmole o NADH i kēlā me kēia minuke ma ka pH 7.6 ma 37 °C