Fructosyl-peptide Oxidase (FPOX)
wehewehe
Pono ka enzyme no ka hoʻoholo ʻana i ka fructosyl-peptide a me ka fructosyl-L-amino acid.
Hoʻolālā Kimia
Kumu Manaʻo
Fructosyl-peptide + H2ʻO + ʻO2→ Peptide + Glucosone + H2O2
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| wehewehe | ʻO ka pauka amorphous keʻokeʻo, lyophilized |
| Ka hana | ≥4U/mg |
| Maʻemaʻe (SDS-PAGE) | ≥90% |
| Catalase | ≤0.01% |
| ATPase | ≤0.005% |
| Glucose oxidase | ≤0.03% |
| Cholesterol oxidase | ≤0.003% |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele: Ambient
Waihona:E kūʻai ma -20°C(Loaʻa), 2-8°C (pōkole)
Ho'āʻo hou ʻiaola:2 makahiki
Moʻolelo hoʻomohala
ʻO kekahi o nā ʻōlelo kuhikuhi i hoʻohana ʻia i ka diagnostics o ka maʻi diabetes he glycated hemoglobin (HbA1c).ʻO ke ana ʻana o ka HbA1c me ka hoʻohana ʻana i nā enzyme kūpono no ka hoʻoponopono ʻana i nā helu he nui, a ʻoi aku ka maikaʻi o ke kumukūʻai.No laila, ua lōʻihi ke kāhea ikaika ʻana mai nā kauka olakino no ka hoʻomohala ʻana i ia ʻano enzyme assay.No laila, ua kūkulu mākou i kahi hōʻike hou me ka hoʻohana ʻana i ka "dipeptide method".Ma ke kiko, ua ʻike mākou i ka "Fructosyl-peptide Oxidase" (FPOX) hiki ke hoʻohana ʻia ma ke ʻano he enzyme no kēia assay.Ua maʻalahi kēia i kā mākou holomua i ka loaʻa ʻana o ka honua mua ma ka hana ʻana i ka ʻoiaʻiʻo o kahi hōʻike enzyme HbA1c.Hoʻohana kēia "dipeptide method" i ka Protease (Proteolytic enzyme) e wāwahi i ka HbA1c i ke kahe koko, a laila e ana i nā pae o nā dipeptides saccharified i hana ʻia me ka FPOX.Ua loaʻa kēia ʻano me ka hoʻokipa maikaʻi loa ʻana ma muli o kāna mau pono o ka maʻalahi, ka maʻalahi a me ka wikiwiki, a ua hoʻohana ʻia ka HbA1c ana reagent e hoʻohana ana i ka FPOX a puni ka honua.
