Homocysteine(HCY)
wehewehe
Hoʻohana ʻia ka Homocysteine (HCY) e ʻike i ka homocysteine i loko o ke koko kanaka.ʻO ka Homocysteine (Hcy) kahi amino acid i loaʻa i ka sulfur i hana ʻia e ka metabolism o ka methionine.Hoʻopaʻa ʻia ka 80% o ka Hcy i nā protein ma o nā paʻa disulfide i loko o ke koko, a he hapa liʻiliʻi wale nō o ka homocysteine manuahi e komo i ke kahe.Pili loa nā pae Hcy i nā maʻi cardiovascular.he kumu pilikia nui ia no ka maʻi cardiovascular.ʻO ka hoʻonui ʻana o ka Hcy i loko o ke koko e hoʻoulu i ka paia o ke kīʻaha koko e hōʻino i ke kīʻaha arterial, e alakaʻi ana i ka mumū a me ka hoʻokumu ʻana o ka plaque ma ka paia o ka moku, a laila e alakaʻi ai i ka paʻa ʻana o ke kahe o ke koko i loko o ka puʻuwai.I nā poʻe maʻi me ka hyperhomocystinuria, hoʻopilikia nā hemahema genetic koʻikoʻi i ka metabolism Hcy, ka hopena i ka hyperhomocysteinemia.ʻO nā hemahema genetic haʻahaʻa a i ʻole nā hemahema o ka meaʻai o nā huaora B e hui pū ʻia me ka haʻahaʻa haʻahaʻa a i ʻole ka haʻahaʻa haʻahaʻa o Hcy, kahi e hoʻonui ai i ka pilikia o ka maʻi puʻuwai.Hiki i ka Hcy kiʻekiʻe ke hoʻoulu i nā kīnā hānau e like me nā defects tube neural a me nā malformations congenital.
Hoʻolālā Kimia
Kumukānāwai hoʻāʻo
Hoʻololi ʻia ʻo Hcy ʻokiʻoki ʻia i Hcy manuahi, a hana ʻo Hcy manuahi me ka serine ma lalo o ka catalysis o CBS e hana ai i ka L-cystathionine.L-cystathionine e hoʻopuka i ka Hcy, pyruvate a me NH3 ma lalo o ka catalysis o CBL.Hiki ke ʻike ʻia ka pyruvate i hana ʻia e kēia hopena pōʻai e ka lactate dehydrogenase LDH a me NADH, a ʻo ka helu hoʻololi ʻana o NADH i NAD e pili pono ana i ka ʻike Hcy i ka hāpana.
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:2-8°C
Ka wā mālama a me ka mana:Pono e mālama i nā reagents i weheʻoleʻia ma 2-8 ° C i ka pōʻeleʻele, aʻo ka manawa kūpono he 12 mahina;ma hope o ka wehe ʻana, pono e mālama ʻia nā reagents i ka pōʻeleʻele ma 2-8 ° C, a ʻo ka manawa kūpono he 1 mahina ma lalo o ke ʻano o ka pollution;ʻAʻole pono e paʻa nā reagents.
Nānā
Nā koi laʻana: ʻO ka laʻana he serum hou a i ʻole plasma (heparin anticoagulation, 0.1mg heparin hiki ke anticoagulate 1.0ml koko).E ʻoluʻolu e centrifuge i ka plasma ma hope koke o ka hōʻiliʻili ʻana i ke koko, a i ʻole e hoʻopaʻa i ka pahu hau a centrifuge i loko o 1 hola.
