Uricase(UA-R) mai Microorganism
wehewehe
He mea maikaʻi kēia enzyme no ka hoʻoholo ʻana i ka lāhui o ka uric acid i ka nānā ʻana.Hoʻokomo ʻo Uricase i ka purine catabolism.Hoʻopili ia i ka hoʻololi ʻana o ka waika uric insoluble i 5-hydroxyisourate.ʻO ka hōʻiliʻili ʻana i ka ʻakika uric ka mea e pōʻino ai ke ake a i ʻole ka maʻi gout.I nā ʻiole, ʻo ka hoʻololi ʻana o ka gene encoding uricase e hoʻonui koke i ka waika uric.ʻO nāʻiole, nele i kēia gene, hōʻike i ka hyperuricemia, hyperuricosuria, a me ka uric acid crystalline obstructive nephropathy.
Hoʻolālā Kimia
Kuʻuna Pane
ʻAkika ʻUric+O2+2H2O→ Allantoin + CO2+ H2O2
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| wehewehe | ʻO ka pauka amorphous keʻokeʻo, lyophilized |
| Ka hana | ≥20U/mg |
| Maʻemaʻe (SDS-PAGE) | ≥90% |
| Solubility (10mg pauka/ml) | Akaka |
| Loaʻa nā enzymes | |
| NADH/NADPH oxidase | ≤0.01% |
| Catalase | ≤0.03% |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:Hoʻouna ʻia ma lalo o -20°C
Waihona :E kūʻai ma -20°C(Loaʻa), 2-8°C(Pokoʻa)
Manaʻo ʻia e hoʻāʻo houola:2 makahiki
huahana pili
E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou
