Cholesterol oxidase(COD/CHOD)
wehewehe
Hoʻopili ka Cholesterol oxidase (CHOD) i ka hana mua o ka cholesterol catabolism.Hiki i kekahi mau bacteria non-pathogenic, e like me Streptomyces ke hoʻohana i ka cholesterol i kumu kalapona.Pono nā bacteria pathogen, e like me Rhodococcus equi, e hoʻopili iā CHOD i ka macrophage o kahi mea hoʻokipa.ʻO ka cholest-5-en-3-one ua isomerized i cholest-4-en3-one. Hiki ke hoʻohuli ʻia ka hopena isomerization.ʻO ka hana o CHOD e pili ana i nā waiwai kino o ka membrane kahi i hoʻopaʻa ʻia ai ka substrate.
Hoʻohana ʻia ʻo CHOD e hoʻoholo i ka cholesterol serum.ʻO ia ka lua o ka enzyme i hoʻohana nui ʻia i nā noi diagnostic ma hope o ka glucose oxidase.Loaʻa iā CHOD ka noi ma ka microanalysis o nā steroids i nā meaʻai a me ka hoʻokaʻawale ʻana i ka 3-ketosteroids mai ka 3b-hydroxysteroids. Ke noiʻi ʻia nei nā mea kanu Transgenic e hōʻike ana i ka cholesterol oxidase i ka hakakā ʻana i ka pulu pulupulu weevil.Ua hoʻohana pū ʻia ʻo Cholesterol oxidase ma ke ʻano he molecular probe e hoʻomaʻamaʻa i nā hale membrane cellular.
Hoʻolālā Kimia
Kumu Manaʻo
Kolekole + O2 →△4-Cholesten-3-one + H2O2
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| wehewehe | ʻO ka pauka amorphous melemele, lyophilized |
| Ka hana | ≥8U/mg |
| Maʻemaʻe (SDS-PAGE) | ≥90% |
| Solubility (10mg pauka/ml) | Akaka |
| Catalase | ≤0.001% |
| Glucose oxidase | ≤0.01% |
| Cholesterol esterase | ≤0.01% |
| ATPase | ≤0.005% |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:Hoʻouna ʻia ma lalo o -15°C
Waihona :E kūʻai ma -25~-15°C(Loaʻa manawa), 2-8°C(Pokoʻa manawa)
Ho'āʻo hou ʻiaola:1 makahiki
