Glucose-6-phosphate dehydrogenase (G6PDH)
wehewehe
ʻO ka hemahema ʻo Glucose 6 phosphate dehydrogenase (G6PD) kahi maʻi hoʻoilina kahi e haki ai ke koko ʻulaʻula (hemolysis) i ka wā e ʻike ʻia ai ke kino i kekahi mau meaʻai, nā lāʻau lapaʻau, nā maʻi a i ʻole ke kaumaha.Loaʻa ia i ka wā e nalowale ai ke kanaka a i ʻole nā pae haʻahaʻa o ka enzyme glucose-6-phosphate dehydrogenase.Kōkua kēia enzyme i ka hana pono ʻana o ke koko ʻulaʻula.ʻO nā hōʻailona i ka wā hemolytic e hiki ke komo i ka mimi ʻeleʻele, ka luhi, ka ʻeleʻele, ka wikiwiki o ka naʻau, ka pōkole o ka hanu, a me ka melemele o ka ʻili (jaundice).Hoʻoili ʻia ka hemahema G6PD ma kahi ʻano hoʻihoʻi X-linked a ʻoi aku ka maʻamau o nā hōʻailona i nā kāne (ʻoi aku ka poʻe ʻAmelika ʻAmelika a me nā poʻe mai kekahi mau wahi o ʻApelika, Asia, a me ke Kaiwaenahonua).Hoʻokumu ʻia ia e nā loli genetic i ka gene G6PD.
ʻO ka Glucose-6-Phophate Dehydrogenase (G-6-PDH) he ʻelua mau ʻāpana o ka paona molekala like. Ua paʻi ʻia ke kaʻina amino acid o ka monomer. nucleotides.Hiki ke ho'āla hou 'ia ka G-6-PDH mai nā hā'ina urea-denatured.
ʻO Glucose 6-phosphate dehydrogenase kahi enzyme hoʻoponopono koʻikoʻi i ka pae mua o ke ala pentose phosphate.Hoʻopiʻi ʻo G-6-P-DH i ka glucose-6- phosphate ma ke alo o NADP+ e hāʻawi i ka 6-phosphogluconate.ʻO ka polyacrylamide gel electrophoresis, ka hoʻopaʻa ʻana i ka hana, a me nā haʻawina anti-yeast G-6-PDH antibody immunoblotting ua hōʻike ʻo G-6-PDH he glycoprotein.
Hoʻolālā Kimia
Kumu Manaʻo
D-Glucose-6-phosphate + NAD+→D-Glucono-δ-lactone-6-phosphate + NADH+H+
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| wehewehe | ʻO ka pauka amorphous keʻokeʻo, lyophilized |
| Ka hana | ≥150U/mg |
| Maʻemaʻe (SDS-PAGE) | ≥90% |
| Solubility (10mg pauka/ml) | Akaka |
| NADH/NADPH oxidase | ≤0.1% |
| Glutathione reductase | ≤0.001% |
| Phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| 6-Phosphogluconate dehydrogenase | ≤0.01% |
| Myokinase | ≤0.01% |
| Hexokinase | ≤0.001% |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele: Ambient
Waihona :E mālama i ka 2-8°C
Ho'āʻo hou ʻiaola:2 makahiki
