ʻO Trypsin hou
Pōmaikaʻi
1. ʻAʻohe kumu holoholona
2.High maemae
3.Better kaʻina hana
4.Stable maikaʻi
wehewehe
Hoʻopau pono ʻo Trypsin i nā paʻa peptide C-terminal o lysine a me arginine, hiki ke hoʻohaʻahaʻa i nā protein paʻa intercellular.ʻO ke kaʻina amino acid o ka trypsin recombinant i hana ʻia e Hzymes Biotech ua like ia me ka trypsin i loaʻa i ka porcine pancreas, a ua hana ʻia e ka recombinant Escherichia coli expression.Hiki iā ia ke hoʻololi i ka unuhi kuʻuna o trypsin no ka hoʻoheheʻe ʻana o nā cell i nā lāʻau lapaʻau, stem cell, immune cell therapy, screening drug, antibodies a me nā kula ʻē aʻe.Hiki i ka Aprotinin, soybean trypsin inhibitor, a pela aku ke kaohi loa i ka hana trypsin.
Loaʻa i ka trypsin recombinant nā waiwai like me ka trypsin i unuhi maoli ʻia.Hana ʻia kēia huahana ma kahi papa hana GMP.ʻAʻole hoʻohana ʻia nā mea ʻai holoholona i ka fermentation, hoʻomaʻemaʻe a me nā mea hope loa.I ka manawa like, ua hōʻoia ʻia nā kaʻina hana hana a me ka hoʻokele maikaʻi, e hopena i kahi kiʻekiʻe o ke kūlike ma waena o nā pūʻulu.
Hoʻolālā Kimia
Wehewehe Unite
25 ℃, pH 7.6, 3.2ml solution solution (1cm ala māmā), hoʻokahi trypsin unit (U) i wehewehe ʻia he
piʻi o 0.003 i ka waiwai absorption ma 253nm e ka enzymatic hydrolysis o BAEE i kēlā me kēia minuke.
Hoohana Huahana
No ka moʻomeheu cell
• ka hoʻoheheʻe ʻia ʻana o ka ʻōpala, ka loaʻa ʻana o ke kelepona mua.
• Ka hoʻoheheʻe ʻana o nā pūnaewele pili.
• Moʻomeheu pūnaewele ma ke ʻano microcarrier.
• E hoʻoheheʻe mālie i nā pūnaewele kumu.
• ʻO ka hoʻomaʻamaʻa kino kino, etc.
No ka protein recombinant
• Hoʻohui hou i ka insulin.
• Ke kaʻina hana protein, ka palapala peptide.
• Nā hana proteolytic kiko'ī e like me ka noiʻi proteomics
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| Ka nana aku | ʻO ka pauka keʻokeʻo a i ʻole ka pauka keʻokeʻo |
| Hoʻoheheʻe (10mg/ml) | Haʻina kala ʻole |
| Maemae | Β-trypsin ≥70%，α-trypsin ≤ 20% |
| Hana Kūikawā (Paka) | ≥2500U/mg |
| E.Coli hoʻokipa protein koena | ≤0.01% |
| Endotoxins(LAL-Ho'āʻo) | ≤20 EU/mg |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:Hoʻouna ʻia ma lalo o 2-8 °C
Waihona:E kūʻai ma -2-8°C, ma hope o ka hoʻoheheʻe ʻia me 1mM hcl a i ʻole 50nM HAC, pono e mālama ʻia ma lalo o -25~-15 °C
Manaʻo ʻia e hoʻāʻo hou i ke ola:2 makahiki
