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Kiʻi Hōʻike ʻia ʻo Trypsin HCP1012A Recombinant
  • Hoʻohui hou ʻo Trypsin HCP1012A

ʻO Trypsin hou


Helu popoki:HCP1012A

Pūʻolo: 0.1g/1g/10g/100g

Hoʻopau pono ʻo Trypsin i nā paʻa peptide C-terminal o lysine a me arginine, hiki ke hoʻohaʻahaʻa i nā protein paʻa intercellular.

Hōʻike huahana

ʻikepili huahana

Hoʻopau pono ʻo Trypsin i nā paʻa peptide C-terminal o lysine a me arginine, hiki ke hoʻohaʻahaʻa i nā protein paʻa intercellular.ʻO ke kaʻina amino acid o ka trypsin recombinant i hana ʻia e Hyasen Biotech ua like ia me ka trypsin i loaʻa i ka porcine pancreas, a ua hana ʻia e ka recombinant Escherichia coli expression.Hiki iā ia ke hoʻololi i ka unuhi kuʻuna o trypsin no ka hoʻoheheʻe ʻana o ka cell i nā lāʻau lapaʻau, stem cell, immune cell therapy, screening drug, antibodies a me nā kula ʻē aʻe.Hiki i ka Aprotinin, soybean trypsin inhibitor, a pela aku ke kaohi loa i ka hana trypsin.

Loaʻa i ka trypsin recombinant nā waiwai like me ka trypsin i unuhi maoli ʻia.Hana ʻia kēia huahana ma kahi papa hana GMP.ʻAʻole hoʻohana ʻia nā mea ʻai holoholona i ka fermentation, hoʻomaʻemaʻe a me nā mea hope loa.I ka manawa like, ua hōʻoia ʻia nā kaʻina hana hana a me ka hoʻokele maikaʻi, e hopena i kahi kiʻekiʻe o ke kūlike ma waena o nā pūʻulu.


  • Mua:
  • Aʻe:

  • Nā hiʻohiʻona huahana

    CAS

    9002-07-7

    EC

    3.4.21.4

    ʻAno

    Mana keʻokeʻo keʻokeʻo keʻokeʻo

    Kaumaha molekula

    24kDa

    kiko isoelectric

    8.26

    Maʻemaʻe (RP-HPLC)

    β-trypsin≥70.0%;α-Trypsin≤20.0%

    Hana Kūikawā

    ≥3800.0U/mg pūmua (BAEE)

    ʻO ka hana trypsin recombinant

    ≥2500.0U/mg mana (BAEE)

    ʻIkepili

    Poni

    ʻO ka pH maikaʻi loa

    8.0

    ʻO ke kaumaha kino

    ≤300CFU/g

    E. Coli host cell protein koena

    ≤0.01%

    E. ʻO Colike koena DNA cell host

    ≤10.0000ng/mg

    Maʻiʻo Endotoxin (LAL-Test)

    ≤20.0EU/mg

    Maʻiʻo protein

    95.0%±10.0%

     

    Nā kūlana mālama

    Paʻa o ka mālama ʻana: Pono e mālama ʻia ka mana recombinant trypsin lyophilized ma lalo o 2-8 ℃.He kūpaʻa i loko o 24 mahina.Ma hope o ka hoʻoheheʻe ʻia me 1mM HCl a i ʻole 50mM HAC, pono e mālama ʻia ma lalo o -25 ~ - 15 ℃.ʻAʻole ia he hana nalo ma hope o 10 mau manawa i ka maloʻo a me ka hoʻolulu.

     

    Wehewehe ʻāpana

    25 ℃ , pH 7.6, 3.2ml reaction solution (1cm ala māmā), hoʻokahi trypsin unit (U) i wehewehe ʻia he piʻi o 0.003 i ka waiwai absorption ma 253nm e ka enzymatic hydrolysis o BAEE i kēlā me kēia minuke.

     

    Ka hoomalu maikai

    Ka hana: Ka hoʻoholo ʻana i ka hana o trypsin e Pharmacopoeia o ka People's Republic of China Volume 4 (2020 Edition).

    .E. ʻO Coli hoʻokipa pūʻali koa pūmua koena: ELISA Kit.Pharmacopoeia of the People's Republic of Kina Volume 4 (2020 Edition) -E. ʻO Coli ke koena pūmua Cell (3412).

    .ʻO ka maʻemaʻe protein: Pharmacopoeia of the People's Republic of Kina Volume 4 (2020 Edition)-Nā Rula Nui no ka Hana HPLC (0512).

    . ʻO ke kaumaha kino: Pharmacopoeia of the People's Republic of Kina Volume 4 (2020 Edition)-Rule General for Sterility Test (1101).E. Colike koena DNA cell hoste: Pharmacopoeia of the People's Republic of Kina Volume 4 (2020 Edition) Host Cell DNA koena (3407).. Endotoxin maʻiʻo: Pharmacopoeia of the People's Republic of Kina Volume 4 (2020 Edition)-Nā Rula Nui no ka LAL-Test (1143).

     

    Aʻo huahana

    1.Hoʻomākaukau no ka hoʻoponopono: E lawe i ka nui kūpono o trypsin e like me ka ʻike protein (helu ʻia e ka hana mana a me ka hana protein) o COA, a hoʻohui i ka solution equilibration HBSS (a i ʻole paʻa ʻē aʻe i kūpono no ka hoʻoheheʻe ʻana o ke kelepona. 0- 1mM, ʻoi aku ka maikaʻi ʻaʻole ma mua o 2mM).ʻO ka manaʻo trypsin i manaʻo ʻia e pili ana i ka 0. 1-0.3mg/mL (hoʻololi ʻia ka concentration e like me nā ʻāpana like ʻole), a hui mālie;hiki ke hana i keia hana ma ka lumi wela.

    2. E hoʻohana i kahi kānana 0.22μm e kānana i ka hopena trypsin a hoʻololi iā ia i kahi pahu sterile;Hiki ke hana ʻia kēia ʻanuʻu ma ka lumi wela.

    3. Pono e hoʻohana pololei ʻia ka hopena trypsin kānana e like me ka mea e pono ai i ka lā (e like me ka hoʻohui ʻana i ka 1mL i nā ʻōmole T25, nā cell digesting ma 37 ° C), a hiki ke mālama ʻia ka wai ma 2-8 ° C no 1-2 pule. .

    4. Ka lōʻihi o ka mālama ʻana: Inā pono e mālama ʻia ka hopena recombinant cell digestion i hoʻomākaukau ʻia no ka manawa lōʻihi, hiki ke mālama paʻa ʻia ma -25~- 15°C no 12 mahina.

     

    Hoʻohana huahana

    No ke kelepona moʻomeheu

    • ka hoʻoheheʻe ʻia ʻana o ka ʻōpala, ka loaʻa ʻana o ke kelepona mua.

    • Ka hoʻoheheʻe ʻana o nā pūnaewele pili.

    • Moʻomeheu pūnaewele ma ke ʻano microcarrier.

    • E hoʻoheheʻe mālie i nā pūnaewele kumu.

    • ʻO ka hoʻomaʻamaʻa kino kino, etc.

    No ka protein recombinant:

    .ʻO ka hana insulin recombinant.

    .ʻO ke kaʻina o ka protein, ka palapala peptide.

    .Nā hana proteolytic kikoʻī e like me ka noiʻi proteomics.

     

    Hoʻomalu

    .Loaʻa ka hopena o ka paʻakai inorganic kiʻekiʻe i ka hana.

    .Hōʻalo i ka pilina me nā huahana protein ʻē aʻe.

    .No kou palekana a me kou olakino, e ʻoluʻolu e hoʻokomo i ka lole lab a me nā mīkina lima no ka operatin

    E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou