Deoxyribonuclease I(Dnase I)
wehewehe
ʻO DNase I (Deoxyribonuclease I) he endodeoxyribonuclease hiki ke ʻeli i ka DNA kaula hoʻokahi a i ʻole ʻelua.Hoʻomaopopo a hoʻokaʻawale i nā mea paʻa phosphodiester e hana i nā monodeoxynucleotides a i ʻole nā oligodeoxynucleotides kaulua a i ʻole me nā hui phosphate ma ka 5'-terminal a me ka hydroxyl ma ka 3'-terminal.Aia ka hana o DNase I ma Ca 2+ a hiki ke ho'āla ʻia e nā ion metala divalent e like me Mn 2+ a me Zn 2+.Mālama ʻo 5 mM Ca 2+ i ka enzyme mai ka hydrolysis.Ma ke alo o Mg 2+, hiki i ka enzyme ke hoʻomaopopo a hoʻokaʻawale i kekahi kahua ma kekahi kaula DNA.Ma ke alo o Mn 2+, hiki ke ʻike ʻia nā kaula ʻelua o ka DNA i ka manawa like a ʻoki ʻia ma kahi kokoke i ka pae hoʻokahi e hana i nā ʻāpana DNA palahalaha a i ʻole nā ʻāpana DNA hope loa me 1-2 nucleotides e puka mai ana.
Hoʻolālā Kimia
Wehewehe Unite
Hoʻokahi ʻāpana i wehewehe ʻia ʻo ka nui o ka enzyme e hoʻohaʻahaʻa loa i 1 µg o pBR322 DNA i 10 mau minuke ma 37°C.
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| Maʻemaʻe (SDS-PAGE) | ≥ 95% |
| Hanana Rnase | ʻAʻohe Hoʻohaʻahaʻa |
| ʻO ka hoʻohaumia gDNA | ≤ 1 kope/μL |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:Hoʻouna ʻia ma lalo o 0 °C
Waihona:E mālama i ka -25~-15°C
Manaʻo ʻia e hoʻāʻo hou i ke ola:2 makahiki
