Uracil DNA Glycosylase (UDG/UNG)
wehewehe
Hoʻopili ka Heat-Labile UDG (uracil DNA glycosylase) i ka hoʻokuʻu ʻia ʻana o ka uracil manuahi mai ka uracil-containing DNA ma o ka hydrolyzing i ka paʻa N-glycoside ma waena o nā kumu uracil a me nā iwi kō-phosphate.Ke hoʻohālikelike ʻia me nā enzymes UDG maʻamau, hiki i ka wela-Labile UDG ke pale i ka hōʻino ʻana o nā huahana amplification dU i hoʻokumu ʻia e ke koena o ka hana o ka UDG inactivated i ka lumi wela.Hana ʻia ka Heat-Labile UDG (uracil DNA glycosylase) ma ka lumi wela a he thermolabile a maʻalahi hoʻi e hoʻopau ʻia.
Hoʻolālā Kimia
Hōʻike
| Enzyme | ʻO ka glycosylase |
| Hoʻopaʻa kūpono | Hoʻopaʻa mālama |
| ʻAʻohe wela | 50°C, 10 min |
| Wehewehe Unite | Hoʻokahi ʻāpana (U) i wehewehe ʻia ʻo ia ka nui o ka enzyme e koi ʻia e hoʻopololei i ka hydrolysis o 1 μg dU-containing dsDNA i loko o 30 mau minuke ma 25°C. |
Nā noi
1.E aʻo i ka hoʻoponopono DNA a me ka ʻike mutation.
2.E hoʻonui i ka pono cloning o nā huahana PCR.
3.E hoʻonui i ka maikaʻi o ka mutagenesis kuhikuhi i ka pae.
Hoʻouna a mālama
Kaʻahele:Puke hau
Kūlana mālama:E kūʻai ma -15 ℃ ~ -25 ℃
Sheef ola:2 makahiki
