ʻO Deoxyribonuclease I hui hou (DNase I, RNase-ʻole)
wehewehe
ʻO ka Recombinant Deoxyribonuclease I, he endonuclease hiki ke hoʻoheheʻe i ka DNA kaula hoʻokahi a i ʻole ʻelua-stranded.ʻO ia ka hydrolyzes phosphodiester paʻa e hana i nā mono- a me nā oligodeoxynucleotides i loaʻa nā pūʻulu 5'-phosphate a me nā pūʻulu 3'-OH.ʻO ka pae pH hana maikaʻi loa o DNase I ʻo 7-8.ʻO ka hana o DNase I e pili ana i Ca2+ a hiki ke hoʻoikaika ʻia e nā ion metala divalent e like me Co2+, Mn2+, Zn2+, a pēlā aku.oiai ma ke alo o Mn2+, DNase hiki ia'u ke oki i ka DNA i kaula-lua ma ka pae hookahi, e hana ana i na welau blunt a i ole 1-2 nuclei Mau hopena me ka nucleotide overhangs.Loaʻa kēia enzyme mai ka recombinant E. coli strains, ʻaʻohe RNase, a hiki ke hoʻohana ʻia no ka hana ʻana i nā ʻano RNA like ʻole.
ʻO ke kinona
Nā noi
RNA extraction: hoʻomākaukau i ka RNA me ka DNA ʻole;
Ma hope o ka transcription in vitro me RNA polymerase, e like me T7 RNA Polymerase (Cat#10618), hoʻohana ʻia e wehe i ka DNA template;
E hoʻomākaukau i ka DNA-free RNA ma mua o RT-PCR a me RT-qPCR;
Hoʻohana pū ʻia me ka DNA Polymerase I (Cat#12903) no ka hoʻopaʻa inoa ʻana i ka DNA ma ka unuhi ʻana i ka nick;
Hoʻohana ʻia i ka nānā ʻana i ka wāwae wāwae e nānā i ka pilina DNA-protein;
E hana i kahi waihona ʻāpana ʻokoʻa;
I ka ʻike ʻana o TUNEL o ka apoptosis, ua hoʻohana ʻia ka DNA genomic i ʻoki ʻia ma ke ʻano he mana maikaʻi.
Hoʻouna a mālama
Kaʻahele:Puke hau
Kūlana mālama:E mālama i loko o -20 ℃
Ke ola papa:2 makahiki
Nā memo:
1. Pono e mālama ʻia nā Enzymes i loko o kahi pahu hau a i ʻole ma kahi ʻauʻau hau ke hoʻohana ʻia, a pono e mālama ʻia ma -20 ° C ma hope koke o ka hoʻohana ʻana.
2. No kou palekana a me kou olakino, e ʻoluʻolu e ʻaʻahu i nā lako pale pilikino (PPE), e like me nā ʻaʻahu hale hana a me nā mīkina lima hoʻolele, i ka wā e hana ai me kēia huahana.
