RT-LAMP Fluorescent Master Mix (Lyophilized Beads)
Hōʻike huahana
Hoʻohana nui ʻia ʻo LAMP i ka ʻenehana i ke kula o ka isothermal amplification.Hoʻohana ia i 4-6 primers hiki ke ʻike i 6 mau ʻāpana kikoʻī ma ka gene i manaʻo ʻia, a hilinaʻi i ka hana hoʻoneʻe strand ikaika o Bst DNA polymerase.Nui nā ʻano hana ʻike LAMP, me ke ʻano kala, ka pH colorimetric method, turbidity method, HNB, calcein, etc. ʻO RT-LAMP kekahi ʻano o ka hopena LAMP me RNA ma ke ʻano he hoʻohālike.ʻO RT-LAMP Fluorescent Master Mix (Lyophilized Powder) ma ke ʻano o ka Lyophilized Powder, a pono e hoʻohui i nā primers a me nā templates i ka wā e hoʻohana ai.
Hōʻike
Nā mea ho'āʻo | Nā kikoʻī |
Endonulease | ʻAʻole i koho ʻia |
hana RNase | ʻAʻohe mea i ʻike ʻia |
Hana DNase | ʻAʻohe mea i ʻike ʻia |
Hana Nickase | ʻAʻohe mea i ʻike ʻia |
E. coli.gDNA | ≤10 kope/500U |
Nā ʻāpana
Aia kēia huahana i ka Reaction Buffer, RT-Enzymes Mix o Bst DNA Polymerase a me Thermotable Reverse Transcriptase, Lyoprotectant a me Fluorescent Dye Components.
Hoʻopili
Hoʻonui Isothermal o DNA a me RNA.
Hoʻouna a mālama
Kaʻahele:Ambient
Kūlana mālama:E mālama i ka -20 ℃
Manaʻo ʻia ka lā hoʻāʻo hou:18 mahina