ʻO Glycohemoglobin A1c (HbA1c) Kiko Hoʻāʻo
Pono
● Kiʻekiʻe pololei
● Hiki ke kū'ē i ka pilikia
● Paʻa maikaʻi
Hoʻolālā Kimia
Nā noi
ʻO ka hoʻāʻo in vitro no ka hoʻoholo ʻana i ka nui o ka HbA1c i ke koko holoʻokoʻa o ke kanaka ma nā ʻōnaehana photometric.ʻO ka HbA1c kahi huahana o ka hemoglobin (Hb) e hoʻopuka ana i ka hopena glycation non-enzymatic ma lalo o ke koko kiʻekiʻe.Hoʻololi ʻo Glucose i ka hemoglobin i loko o kāna koena valine n-terminal e hana i ka hemoglobin glycated.Ma lalo o nā kūlana physiological maʻamau, ʻoi aku ka maikaʻi o ka hana ʻana o nā huahana glycosylation non-enzymatic i ka ʻike o nā reactants.Ma muli o ka paʻa ʻana o ka pae o ka hemoglobin, pili ka nui o ka glycosylation i ka ʻike glucose a pili pū kekahi i ka lōʻihi o ka hemoglobin a me ka ʻike glucose.No laila, he hōʻailona maikaʻi ʻo HbA1c i ka pae koko glucose awelika o nā maʻi no nā mahina 2-3 i hala.
Kumukānāwai
Ma lalo o ka hana o ka protease, ua ʻoki ʻia ka n-terminal o ke kaulahao β i HbA1c a hoʻokuʻu ʻia nā dipeptides glycosylated.I ka hopena mua, hiki ke loaʻa ka ʻike Hb ma ke ana ʻana i ka absorbance o 480 nm.I ka lua o ka hopena, hana ʻo fructosyl peptide oxidase (FPOX) i nā dipeptides glycosylated e hoʻohua i ka hydrogen peroxide i hiki ke hana me nā mea chromogenic e hoʻohua ai i ka absorbance ma 660nm i mua o ka peroxidase, a laila hiki ke loaʻa ka ʻike o ka HbA1c ma ke ana ʻana i ka absorbance o. 660nm.E like me ka loaʻa ʻana o ka HbA1c a me ka Hb, hiki ke helu ʻia ka pākēneka o HbA1c(HbA1c%).
Pili
ʻO Hitachi 7180/7170/7060/7600 mea hoʻoponopono biochemical maʻalahi, Abbot 16000, OLYMPUS AU640 mea hoʻoponopono biochemical maʻalahi.
Nā mea hoʻopaʻa
| Nā ʻāpana | Hoʻopaʻa ʻana |
| Mea hoʻopili 1(R1) | |
| Paipai maikaʻi | 100mmol/L |
| PRK | 500KU/L |
| DA-67 | 10mmol/L |
| 2 (R2) | |
| Paipai maikaʻi | 100mmol/L |
| ʻO Fructosyl peptide oxidase | 50 KU/L |
| Mea hoʻopili 3(R3) | |
| Paipai maikaʻi | 100mmol/L |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:Ambient
Ka mālama ʻana a me ke kūpaʻa:
A hiki i ka lā pau i hōʻike ʻia ma ka lepili, ke mālama ʻia me ka wehe ʻole ʻia ma 2-8 ℃ a pale ʻia mai ka mālamalama.Ke wehe ʻia, paʻa nā reagents no 28 mau lā i ka wā i hoʻopaʻa ʻia i ka mea ananaly a i ʻole ka friji.
Pono e pale i ka haumia o nā reagents.Mai hoʻokuʻu i nā reagents.
Ke hoʻoheheʻe ʻia, paʻa ka calibrator no 15 mau lā ma 2-8 ℃, paʻa ka mana no 7 mau lā ma 2-8 ℃, mai maloʻo.
Ke ola papa:1 makahiki
