Hoʻokahi ʻanuʻu RT-qPCR pahu pahu
wehewehe
Hoʻolālā kūikawā ʻia ʻo One Step qRT-PCR Probe Kit no qPCR e hoʻohana pololei ana i ka RNA (eg virus RNA) ma ke ʻano he laʻana.Me ka hoʻohana ʻana i nā gene specific primers (GSP), hiki ke hoʻopau i ka transcription reverse a me qPCR i hoʻokahi paipu, e hōʻemi nui ana i nā kaʻina pipetting a me ka pilikia o ka hoʻohaumia ʻana.Hiki ke hoʻopau ʻia i ka 55 ℃ me ka ʻole e pili ana i ka pono a me ka naʻau o qRT-PCR.Hoʻokahi ʻanuʻu qRT-PCR Probe Kit i hāʻawi ʻia ma Master Mix.Aia i loko o ka 5 × One Step Mix kahi buffer optimized a me dNTP/dUTP Mix, a he kūpono ia no nā ʻōnaehana ʻike kikoʻī kiʻekiʻe e pili ana i nā probes lepili fluorescence (e laʻa me TaqMan).
Nā Kumu Kumu o RT-qPCR
Ka wehewehe
| Nā mea ho'āʻo | Hōʻike | Ka hopena |
| (SDS PAGE) Ma'ema'e o ka Enzyme Stock(SDS PAGE) | ≥95% | Holo |
| Endonuclease Activity | ʻAʻole ʻike ʻia | Holo |
| Ka hana Exodulease | ʻAʻole ʻike ʻia | Holo |
| Hanana Rnase | ʻAʻole ʻike ʻia | Holo |
| Ke koena E.coli DNA | ＜1 kope/60 | Holo |
| Pūnaehana ʻIke Hana Hana | 90%≤110% | Holo |
Nā ʻāpana
| Nā ʻāpana | 100rxns | 1,000rns | 5,000 rxns |
| RNase-noa ddH2O | 2*1ml | 20ml | 100ml |
| 5 * hoʻokahi ʻanuʻu hui | 600μl | 6*1ml | 30ml |
| Hoʻokahi ʻanuʻu hui enzyme | 150μl | 2*750μl | 7.5ml |
| 50* Hōʻike ROX Peila 1 | 60μl | 600μl | 3*1ml |
| 50* Hōʻike ROX Peila 2 | 60μl | 600μl | 3*1ml |
a.Hoʻokahi-Step Buffer me ka dNTP Mix a me Mg2+.
b.Aia i loko o ka Enzyme Mix ka hoʻohuli
transcriptase, Hot Start Taq DNA polymerase (hoʻololi antibody) a me ka mea hoʻopaneʻe RNase.
c.Hoʻohana ʻia e hoʻoponopono i ka hewa o nā sinals fluorescene ma waena o nā pūnāwai like ʻole.
d.ROX: Pono ʻoe e koho i ka calibration e like me ke kumu hoʻohālike o ka mea hoʻāʻo.
Nā noi
ʻIke QPCR
Hoʻouna a mālama
Hoʻouna:Puke hau
Kūlana mālama:E mālama i ka -20 ℃.
Shief Life:18 mahina
