Peroxidase (Horseradish source) Synonym: Hydrogen peroxide oxidoreductase;HRP

Hoʻokaʻawale ʻia ka Horseradish peroxidase (HRP) mai nā aʻa o ka horseradish (Amoracia rusticana) a no ka hui ferroprotoporphyrin o peroxidases.Hoʻohui koke ʻo HRP me ka hydrogen peroxide (H2O2).Hiki i ka paʻakikī [HRP-H2O2] ke hoʻoneʻe i nā ʻano mea hāʻawi hydrogen:
Hāʻawi + H2O2 → Mea Hāʻawi Oxidized + 2 H2O
Na HRP e oxidize i nā substrate like ʻole (e nānā i ka Papa 1):
• Chromogenic
• Kemiluminescent (e like me ka luminol a i ʻole isoluminol)
• Fluorogenic (e like me tyramine, homovanillic acid, a i ʻole 4-hydroxyphenyl acetic acid)
ʻO HRP kahi polypeptide kaulahao hoʻokahi i loaʻa nā alahaka disulfide ʻehā.ʻO ka HRP kahi glycoprotein i loaʻa i ka 18% carbohydrate.ʻO ka hui ʻana o ka carbohydrate he galactose, arabinose, xylose, fucose, mannose, mannosamine, a me galactosamine, e pili ana i ka isozyme kikoʻī.

He lepili nui ka HRP no nā immunoglobulins i nā noi immunochemistry like ʻole, me ka immunoblotting, immunohistochemistry, a me ELISA.Hiki ke hoʻohui ʻia ka HRP i nā antibodies e nā ʻano ʻano like ʻole, me ka glutaraldehyde, periodate oxidation, ma o nā paʻa disulfide, a ma o ka amino a me ka thiol directed cross-linkers.ʻO HRP ka lepili i makemake nui ʻia no nā antibodies, ʻoiai ʻo ia ka liʻiliʻi a paʻa loa o nā lepili enzyme kaulana ʻekolu (peroxidase, β-galactosidase, alkaline phosphatase) a me kāna glycosylation e alakaʻi i ka hoʻopaʻa haʻahaʻa ʻole.Ua paʻi ʻia kahi loiloi o ka glutaraldehyde a me nā ʻano conjugation periodate.

Hoʻohana ʻia ʻo Peroxidase no ka hoʻoholo ʻana i ka glucose4 a me nā peroxides i ka hopena.Nui nā puke, 6-24 theses, 25-29 a me nā dissertation 30-46 i hōʻike i ka hoʻohana ʻana o P8375 i kā lākou mau noiʻi noiʻi.