Poly A lawe RNA
Helu popoki: HC4001A
ʻO Poly A, polyadenylate, kahi hui o 100 ~ 10000 polyadenylates, kahi i polymerized e polynucleotide phosphorylase in vitro.I loko o vivo, hoʻohui ʻia ka poly (a) i ka 3-terminal o ka mRNA e ka enzyme e hoʻomaikaʻi i ka paʻa o ka mRNA.I ka hoʻohana ʻana i ka ʻokiʻoki nucleic acid, hoʻohui i ka poly A i ka lysate a i ʻole ka hopena paʻa e hiki ke hoʻomaikaʻi i ka hua o DNA a me RNA.ʻO ka mīkiniʻO ka poly A e hoʻomaikaʻi i ka hua o ka nucleic acid penei:
1. Hoʻopili piha me ka adsorption ili o nā ʻatikala.ʻO ka hapa nui o nā ʻatikala polypropylene he uila paʻa ma ka ʻili, kahi e hoʻopili ai i nā waikawa nucleic.Hiki i ka mea lawe RNA ke hoʻopiha i kēia mau meahopena adsorption a hoʻemi i ka nalowale o nā ʻakika nucleic target.
2. Inactivate trace nucleases: Aia nā ʻano nucleases i nā laʻana olaola akaiapuni.Hiki i ka Poly A ke hoʻopau i nā trace nucleases i ka ʻohi ʻana a i ʻole ka mālama ʻana i nā ʻanuʻu ihoʻomaikaʻi i ka hua a me ka paʻa o nā ʻakika nucleic target.
3. Coprecipitation: Ma ke kaʻina hoʻomaʻemaʻe nucleic acid o ka ua i hoʻopaʻa ʻia i ka waiʻona a i ʻole ka hoʻopaʻa ʻana, hiki i ka poly A ke hui pū me ka waikawa nucleic target a i ʻole e hana i nā ʻāpana polymer ihoʻomaikaʻi i ka hoʻōla.
Nā kūlana mālama
-20 ~ 8 ℃, mālama maloʻo, mālama mālama lōʻihi i -20 ℃
Hōʻike
| Helu CAS | 26763-19-9 |
| Ka nana aku | ʻO ka pauka lyophilized keʻokeʻo |
| Maemae | 99% |
| Kaumaha Molekala | 700-3500 KDa |
ʻano hoʻohana
E lawe i ka nui kūpono o ka pauka lyophilized, hoʻohui i ka wai i mālama ʻia DEPC a i ʻole ka wai paʻakai guanidinee hoʻoheheʻe i loko o 0.1-1ug/uL, a laila e hoʻopaʻa i lalo a mālama iā ia ma -20 ° C.
Nā noi
1.Virus DNA / RNA extraction: hoʻohui i ka 1-5ug Carrier RNA i ka lysate hiki ke hoʻomaikaʻi i ka hua o ka RNA / DNA, hoʻopaʻa i ka waikawa nucleic target a pale i ka hoʻohaʻahaʻa ʻana o ka nucleic acid i ka wā mālama.
2. I loko o ka micro DNA / RNA extraction ma ke kolamu membrane method (<1ug), hoʻohui i ka carrier RNA i 1-5ug mea kūpono e hoʻomaikaʻi i ka hua o ka nucleic acid.
3. I loko o ka waiʻona i hoʻopili ʻia i ka wai nucleic acid precipitation a me ka pae kuʻuna, ʻo ka hoʻohui ʻana o 1-2ug carrier RNA he mea kōkua e hoʻomaikaʻi i ka hoʻihoʻi ʻana o ka RNA māhele pōkole.
4. I loko o ka quantitative probe PCR reaction solution, hoʻohui i ka 10-100ng carrier RNA i ka hopena hopena he mea kōkua ia e hoʻomaikaʻi i ka naʻau a hoʻemi i ka C.Twaiwai.
