Ribonuclease III (RNase III)
wehewehe
ʻO kēia huahana he ribonuclease III (RNase III) i hōʻike hou ʻia e E. coli.Hoʻopau kēia exonuclease kikoʻī i ka RNA (dsRNA) pālua ʻelua a hana i nā ʻāpana 12-35bp dsRNA me 5'-PO4 a me 3'-OH, 3' overhangs
Hoʻolālā Kimia
Wehewehe Unite
Wehewehe ʻāpana hana: Hoʻokahi ʻāpana e pili ana i ka nui o ka enzyme e pono ai e hoʻohaʻahaʻa i 1 μg o
dsRNA i ka siRNA i loko o kahi ʻōnaehana hopena 50 μL ma 37 ° C no 20 mau minuke.
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| Ka hana Exonuclease | Hoʻokuʻu < 0.1% o ka huina radioactivity |
| ʻO ka hana Dnase Kūikaʻi ʻole | ʻAʻole ʻike ʻia |
| Hoʻomaʻemaʻe Protein (SDS-PAGE) | ≥ 95% |
| ʻO ka hana RNase | ＞90% o ka substrate RNA e mau ana |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:Hau maloo
Waihona:E mālama i ka -25~-15°C
Manaʻo ʻia e hoʻāʻo hou i ke ola:2 makahiki
huahana pili
E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou
