RT-LAMP Colormetric (Polo Lyophilized)
Aia i loko o kēia huahana ka mea ho'ōla pane, RT-Enzymes Mix (Bst DNA polymerase a me ka wela-resistant reverse transcriptase), nā mea pale lyophilized a me nā ʻāpana dye chromogenic.ʻO ka huahana he lyophilized ball type, hoʻohana wale me nā primers a me nā templates.Hāʻawi kēia pahu i kahi ʻike wikiwiki a maopopo o ka amplification, ʻo ia ka hopena maikaʻi ʻole i hōʻike ʻia i ka ʻulaʻula a hōʻike ʻia ka hopena maikaʻi e ka hoʻololi ʻana i ka melemele.
ʻāpana
RT-LAMP Colormetric Master Mix (Lyophilized beads)
Nā noi
No ka DNA a i ʻole RNA isothermal amplification.
Nā Kūlana Waihona
Lawe ʻia a mālama ʻia ma 2 ~ 8 ℃.Pono ka huahana no 12 mahina.
Kūkākūkā
1.E lawe i ka pauka Lyophilized beads e like me ka helu o nā hoʻokolohua.
2.E hoʻomākaukau i ka hui ʻana
| ʻāpana | Volume |
| RT-LAMP Colormetric Master Mix (Lyophilized beads) | 1 ʻāpana (2 lei) |
| 10 × Hui Pūmuaa | 5 μL |
| Nā templates DNA/RNA b | 45 μL |
Nā memo:
1. 10×Primer Mix Concentration: 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;
2. Manaʻo ʻia e hoʻoheheʻe ʻia nā mamana nucleic acid me ka wai DEPC.
3.Incubate ma 65 ° C no 30-45mins, hiki ke hoʻolōʻihi ʻia e like me ka hoʻololi kala ʻana.
4.Wahi a ka maka ʻōlohelohe, maikaʻi ka melemele a maikaʻi ʻole ka ʻulaʻula.
Nā memo
1.Hiki ke hoʻonui ʻia ka mahana hopena ma waena o 62 ℃ a me 68 ℃ e like me ke kūlana mua.
2.ʻAʻole pono e ʻike ʻia nā reagents i hoʻopaʻa ʻia i ka ea no ka manawa lōʻihi.
3.Hoʻololi ka ʻulaʻula a melemele i ka hoʻololi ʻana o ka pH o ka ʻōnaehana hopena, e ʻoluʻolu mai hoʻohana i ka Tris nucleic acid storage solution, ʻōlelo ʻia e hoʻohana i ka ddH2ʻO ka waika nucleic mālama ʻia.
4.E mālama ʻia ka hoʻokolohua ma ke ʻano maʻamau, me ka hoʻomākaukau ʻana i ka ʻōnaehana pane, ka laʻana lapaʻau a me ka hoʻohui ʻana.
5.Manaʻo ʻia e hoʻomākaukau i ka ʻōnaehana pane ʻana i ka papaʻaina ultra-clean a hoʻohui i nā mamana i loko o ka puʻupuʻu o nā lumi ʻē aʻe e pale aku i ka hoʻopunipuni.
