Uracil DNA Glycoylase (Glycerol-free)

No CAS: 39369-21-7 EC no.: 3.2.2.27

Pūʻolo: 100U, 500U, 5KU, 10KU

Hōʻike huahana

wehewehe

Hiki i ka Thermosensitive UDG (uracil-DNA glycosylase) ke hoʻokahe i ka hydrolysis o ke kumu uracil o ke kaulahao DNA i loaʻa ka uracil a me ka paʻa N-glycosidic o ka iwi kuamoʻo kō-phosphate e hoʻokuʻu i ka uracil manuahi.Hoʻohālikelike ʻia me nā enzymes UDG maʻamau, ʻaʻole nā enzyme UDG thermosensitive i ke koena o ka hana ʻana o nā enzyme UDG maʻamau ma hope o ka hana ʻole ʻana, hiki ke hoʻohaʻahaʻa i nā huahana amplification dU i loko o ka lumi wela.Ke hana nei kēia huahana ma ka lumi wela a he maʻalahi ka wela a hiki ke hana ʻole.

Hoʻolālā Kimia

Hōʻike

Enzyme	ʻO ka glycosylase
Hoʻopaʻa kūpono	Hoʻopaʻa mālama
ʻAʻohe wela	50°C, 10 min
Wehewehe Unite	Hoʻokahi ʻāpana (U) i wehewehe ʻia ʻo ia ka nui o ka enzyme e koi ʻia e hoʻokaʻawale i ka hydrolysis o 1 μg dU-containing dsDNA i loko o 30 mau minuke ma 25°C.