ʻO ka pahu hoʻoheheʻe DNA/RNA maʻiʻo
He mea kūpono kēia kit no ka unuhi wikiwiki ʻana o ka DNA/RNA viral maʻemaʻe kiʻekiʻe mai nā laʻana e like me ka nasopharyngeal swabs, nā ʻōpala kaiapuni, nā supernatants moʻomeheu cell, a me nā supernatants homogenate tissue.Hoʻokumu ʻia ka pahu i ka ʻenehana hoʻomaʻemaʻe silica membrane e hoʻopau i ka pono no ka hoʻohana ʻana i nā mea hoʻoheheʻe organik phenol/chloroform a i ʻole ka wai inu ʻawaʻawa e hoʻopau i ka DNA / RNA o ke ʻano kiʻekiʻe.ʻO nā ʻakika nucleic i loaʻa ʻaʻole i haumia a mākaukau no ka hoʻohana ʻana i nā hoʻokolohua o lalo e like me ka reverse transcription, PCR, RT-PCR, PCR manawa maoli, next-generation sequencing (NGS), a me Northern blot.
Nā kūlana mālama
E kūʻai ma 15 ~ 25 ℃, a lawe i ka lumi wela
Nā ʻāpana
| Nā ʻāpana | 100RXNS |
| Hoʻopaʻa VL | 50 ml |
| Hoʻopaʻa RW | 120 ml |
| RNase-noa ddH2 O | 6 ml |
| Nā Kolu RNA FastPure | 100 |
| Nā Paipu Ohi (2ml) | 100 |
| Nā Paipu Ohi ʻAʻohe RNase (1 .5ml) | 100 |
Hoʻopaʻa VL:Hāʻawi i kahi kaiapuni no ka lysis a me ka hoʻopaʻa ʻana.
Hoʻopaʻa RW:Wehe i nā protein koena a me nā mea haumia ʻē aʻe.
RNase-noa ddH2O:E hoʻokaʻawale i ka DNA/RNA mai ka membrane ma ke kolamu milo.
Nā Kolu RNA FastPure:Hoʻopili pono i ka DNA/RNA.
ʻO nā paipu hōʻiliʻili 2 ml:E hōʻiliʻili i ka kānana.
Nā Paipu Kohi ʻAʻohe RNase 1.5 ml:E hōʻiliʻili i ka DNA/RNA.
Nā noi
Nasopharyngeal swabs, environmental swabs, cell culture supernatants, and tissue homogenate supernatants.
Mater hoʻomākaukau ponoʻīials
ʻO nā ʻōlelo aʻoaʻo paipu ʻole RNase, 1.5 ml RNase-free centrifuge paipu, centrifuge, vortex mixer, a me nā paipu.
Kaʻina hoʻokolohua
Hana i nā ʻanuʻu a pau i loko o kahi keʻena palekana biosafety.
1. Hoʻohui i 200 μl o ka hāpana i ka RNase-free centrifuge tube (make up me PBS a i ʻole 0.9% NaCl inā lawa ʻole ka hāpana), e hoʻohui i 500 μl o Buffer VL, e hui maikaʻi ma ka vortexing no 15 - 30 sec, a me ka centrifuge. pōkole e hōʻiliʻili i ka hui ʻana ma lalo o ka paipu.
2. E kau i nā kolamu RNA FastPure i loko o kahi pahu ohi 2 ml.E hoʻololi i ka hui ʻana mai ka Step 1 a i FastPure RNA Columns, centrifuge ma 12,000 rpm (13,400 × g) no 1 min, a hoʻolei i ka kānana.
3. E hoʻohui i 600 μl o Buffer RW i FastPure RNA Columns, centrifuge ma 12,000 rpm (13,400 × g) no 30 sec, a hoʻolei i ka kānana.
4. E hana hou i ka ʻanuʻu 3.
5. E centrifuge i ke kolamu hakahaka ma 12,000 rpm (13,400 × g) no 2 min.
6. E hoʻololi pono i nā kolamu FastPure RNA i loko o ka RNase-free Collection Tubes 1.5 ml (i hāʻawi ʻia i loko o ka pahu), a hoʻohui i 30 - 50 μl o RNase-free ddH2O i ke kikowaena o ka membrane me ka hoʻopā ʻole i ke kolamu.E ʻae e kū ma ka lumi wela no 1 min a me ka centrifuge ma 12,000 rpm (13,400 × g) no 1 min.
7. E hoʻolei i nā kolamu RNA FastPure.Hiki ke hoʻohana pololei ʻia ka DNA/RNA no nā hoʻokolohua ma hope, a mālama ʻia ma -30 ~ -15 ° C no kahi manawa pōkole a i ʻole -85 ~ -65 ° C no kahi manawa lōʻihi.
Nā memo
No ka noiʻi wale nō.ʻAʻole no ka hoʻohana ʻana i nā kaʻina diagnostic.
1. E hoʻohālikelike i nā laʻana i ka lumi wela ma mua.
2. He ma'i ma'i ma'i.E ʻoluʻolu e hōʻoia i ka mālama ʻia ʻana o nā pale palekana āpau ma mua o ka hoʻokolohua.
3. E hō'ole i ka ho'ohau a ho'ohehe'e pinepine 'ia o ka hāpana, no ka mea, hiki i kēia ke ho'oha'aha'a a emi mai paha ka hua o ka DNA/RNA viral i unuhi 'ia.
4. ʻO nā mea hana hoʻomākaukau ponoʻī e pili ana i nā ʻōlelo aʻoaʻo pipette RNase-free, 1.5 ml RNase-free centrifuge paipu, centrifuge, vortex mixer, a me nā pipettes.
5. I ka wā e hoʻohana ai i ka pahu, e ʻaʻahu i ka lole keʻena hana, nā mīkina lima latex i hoʻopau ʻia, a me ka pale maka a hoʻohana i nā mea hoʻohana ʻole RNase e hōʻemi i ka pilikia o ka haumia o RNase.
6. Hana i nā ʻanuʻu a pau ma ka lumi wela ke ʻole i kuhikuhi ʻia.
Mechanism & Workflow
