ʻO ka Virus DNA/RNA Extraction Kit
Hiki i ke kit(HC1009B) ke wehe koke i nā waika nucleic viral maʻemaʻe kiʻekiʻe (DNA/RNA) mai nā ʻano wai like ʻole e like me ke koko, serum, plasma, a me ka wai holoi swab, e hiki ai i ka hoʻoili kiʻekiʻe-throughput o nā laʻana like.Ke hoʻohana nei ka pahu i nā peʻa magnetic superparamagnetic silicon-based magnetic beads.I loko o kahi ʻōnaehana pale kūʻokoʻa, hoʻopili ʻia nā waikawa nucleic ma kahi o nā protein a me nā mea haumia ʻē aʻe e nā paʻa hydrogen a me ka hoʻopaʻa electrostatic.Holoi ʻia nā peʻa magnetic i hoʻopili ʻia i nā waikawa nucleic e wehe i nā protein a me nā paʻakai i koe.I ka hoʻohana ʻana i ka paʻakai paʻakai haʻahaʻa, hoʻokuʻu ʻia nā nucleic acid mai nā peʻa magnetic, i mea e hoʻokō ai i ke kumu o ka hoʻokaʻawale wikiwiki a me ka hoʻomaʻemaʻe ʻana i nā waikawa nucleic.ʻO ke kaʻina hana holoʻokoʻa he maʻalahi, wikiwiki, palekana a maikaʻi hoʻi, a hiki ke hoʻohana pololei ʻia nā nucleic acids no nā hoʻokolohua downstream e like me ka reverse transcription, PCR, qPCR, RT-PCR, RT-qPCR, sequencing next-generation, biochip analysis, etc.
Nā kūlana mālama
E kūʻai ma 15 ~ 25 ℃, a lawe i ka lumi wela.
Nā noi
ʻO ke koko, serum, plasma, swab eluent, tissue homogenate a me nā mea hou aku.
Kaʻina hoʻokolohua
1. Laʻana hana ʻana
1.1 No nā maʻi maʻi i loko o nā ʻano wai e like me ke koko, serum, a me ka plasma: 300μL o supernatant i hoʻohana ʻia no ka unuhi ʻana.
2.2 No nā laʻana swab: E kau i nā laʻana swab i loko o nā paipu laʻana i loaʻa ka hopena mālama, vortex no 1 min, a lawe i 300μL supernatant no ka unuhi ʻana.
1.3 No nā maʻi maʻi i loko o nā homogenates kiko, nā hāʻina tissuesoak, a me nā laʻana kaiapuni: Kū i nā laʻana no 5 -10 min, a lawe i 300μL o supernatant no ka unuhi ʻana.
2. Ka hoomakaukau ana o hoʻomākaukauackaged reagent
Wehe i nā reagents i hoʻopaʻa mua ʻia mai ka pahu, hoʻohuli a hoʻohui i nā manawa he nui e hoʻopaʻa hou i nā lei magnetic.E lulu mālie i ka pā i mea e pohō ai nā reagents a me nā peʻa magnetic i lalo o ka pūnāwai.E ʻoluʻolu e hōʻoia i ke kuhikuhi o ka pā a e uhae pono i ka sila alumini pepa.
Δ Hōʻalo i ka haʻalulu i ka wā e haehae ai i ke kiʻi hoʻopaʻa i mea e pale ai i ke kahe ʻana o ka wai.
3. Hana o ka kaamea kani atic
3.1 Hoʻohui i 300μL o ka laʻana i nā pūnāwai ma nā kolamu 1 a i ʻole 7 o ka pā punawai hohonu 96 (e nānā pono i ke kūlana hana maikaʻi).ʻO ka nui hoʻokomo o ka laʻana ua kūpono me 100-400 μL.
3.2 E hoʻokomo i ka pā punawai hohonu 96 i loko o ka mea unuhi ʻakika nucleic.E hoʻokomo i nā lima lima pahu magnetic, a e hoʻopaʻa pono iā lākou e hoʻopuni piha i nā koʻokoʻo magnetic.
3.3 E hoʻonoho i ka polokalamu penei no ka unuhi ʻakomi:
3.4 Ma hope o ka unuhi ʻana, e hoʻololi i ka eluent mai nā kolamu 6 a i ʻole 12 o ka pā punawai hohonu 96 (e nānā pono i ke kūlana hana maikaʻi) i kahi paipu centrifuge maʻemaʻe Nuclease-free.Inā ʻaʻole ʻoe e hoʻohana koke, e ʻoluʻolu e mālama i nā huahana ma -20 ℃.
Nā memo
No ka noiʻi wale nō.ʻAʻole no ka hoʻohana ʻana i nā kaʻina diagnostic.
1. ʻO DNA/RNA ka huahana i unuhi ʻia.Pono e uku nui ʻia e pale i ka hōʻino ʻana o RNA e RNase i ka wā o ka hana.Pono e hoʻolaʻa ʻia nā ipu a me nā mea hoʻohālike i hoʻohana ʻia.Pono e ho'oma'ema'e 'ia nā paipu a me ka pipette a me ka DNase/RNase-noa.Pono nā mea hana e hoʻokomo i nā mīkina lima pauka a me nā masks.
2. E ʻoluʻolu e heluhelu pono i ka manual aʻoaʻo ma mua o ka hoʻohana ʻana, a e hana e like me ka palapala kuhikuhi.Pono e hana ʻia ka hana laʻana i loko o kahi papa hoʻomaʻemaʻe ultra a i ʻole kahi keʻena palekana olaola.
3. Pono e ho'oma'ema'e 'ia ka 'ōnaehana nucleic acid e UV no 30 min ma mua a ma hope o ka ho'ohana 'ana.
4. Loa'a paha nā meheu o nā pe'a magnetic i koe i loko o ka eluent ma hope o ka unuhi'ana, no laila e pale aku i ka ho'omo'o 'ana i nā pe'a magnetic.Inā hoʻomohu ʻia nā lei magnetic, hiki ke hoʻoneʻe ʻia me kahi kūpaʻa magnetic.
5. Inā ʻaʻohe ʻōlelo aʻoaʻo kūikawā no nā ʻāpana like ʻole o nā reagents, e ʻoluʻolu e hoʻohui iā lākou, a e hōʻoia e hoʻohana ʻia nā kits i loko o ka manawa kūpono.
6. E hoʻolei pono i nā laʻana a me ka reagent, holoi pono a hoʻopau i nā wahi hana me 75% ethanol.
