2×HiF Taq me Master Mix

Helu popoki: HCR2014B

ʻO HIF Taq me Master Mix (Me ka Dye) kahi hoʻonā 2×premixed mākaukau e hoʻohana i loaʻa ka Plus HIF DNA Polymerase, dNTPs, a me ka buffer i hoʻopaʻa ʻia.Elua monoclonal antibodies i ka lumi wela e kaohi ana i ka hana polymerase a me ka 3′→5′exonuclease hana i hookomoia i ka master mix no ka Hot Start PCR maʻalahi.Hoʻohui ʻia ka mea hoʻonui i ka master mix e hāʻawi i ka enzyme i kahi hiki ke hoʻonui i ka ʻāpana ʻāpana lōʻihi, hiki i ka lōʻihi o ka hoʻonui ʻana ke piʻi i ka 13 kb, ʻo ka enzyme he 5′→3′ DNA polymerase hana a me kahi 3′→5′. ʻO ka hana exonuclease, ʻo kona kūpaʻa he 83 mau manawa ma mua o Taq DNA polymerase, ʻo ia ka 9 mau manawa o ka polymerase DNA maʻamau.He kūpono ia no ka hoʻonui ʻana i nā template paʻakikī, ʻo ka huahana amplification he hopena blunt.

2×HIF Taq plus Master Mix(Me ka Dye) loaʻa nā pōmaikaʻi o ka wikiwiki a me ka maʻalahi, ka naʻau kiʻekiʻe, ka kikoʻī ikaika, ka paʻa maikaʻi, a me nā mea ʻē aʻe, pono ka ʻōnaehana hopena e hoʻohui i nā primers a me nā templates, a hiki ke hoʻonui ʻia e kahi ʻelua- kaʻina protocol, hoʻomaʻamaʻa i nā ʻanuʻu hoʻokolohua a mālama i ka manawa.Loaʻa i kēia huahana nā mea hoʻonaninani electrophoresis, a hiki ke hoʻohana pololei ʻia nā huahana PCR no ka electrophoresis.Eia kekahi, loaʻa i ka huahana kahi mea pale pono, i hiki i ka master mix ke mālama i ka hana paʻa ma hope o ka hoʻoheheʻe pinepine ʻana.

Nā Kūlana Waihona

Pono e mālama ʻia nā huahana ma -25 ~ -15 ℃ no 1 makahiki.

Nā kikoʻī

Nā kuhikuhi

1.Pūnaehana hoʻololi PCR

2.Manaʻo ʻia ka hoʻohana ʻana i nā mamana like ʻole

3.Hoʻolaha Hoʻolaha

1) ʻElua-Step Protocol (meʻa paʻakikī)

2) ʻEkolu-Step Protocol (kuʻuna maʻamau)

3) Anaaling Gradient Protocol (ka hoʻohālikelike paʻakikī)

Nā hiʻohiʻona ma lalo o nā protocol amplification like ʻole

Nā memo

E ʻoluʻolu e ʻaʻahu i ka PPE pono, e like me ka lole lab a me nā mīkina lima, e hōʻoia i kou olakino a palekana!