Bst 2.0 DNA Polymerase (Glycerol free, high density)
Loaʻa ka Bst DNA polymerase V2 mai Bacillus stearothermophilus DNA Polymerase I, nona ka hana 5′→3′ DNA polymerase a me ka hana hoʻololi kaulahao ikaika, akā ʻaʻohe 5′→3′ exonuclease hana.He kūpono loa ka Bst DNA Polymerase V2 no ka hoʻoneʻe ʻana i ke kaula, ka LAMP hoʻonui isothermal (Loop mediated isothermal amplification) a me ke kaʻina wikiwiki.Hiki i kēia Bst DNA polymerase V2 ke kāohi i ka hana DNA polymerase ma ka lumi wela, i hiki ke hana ʻia a hiki ke hoʻonohonoho ʻia ka ʻōnaehana hopena i ka lumi wela, e pale ana i ka hoʻonui ʻole ʻana a me ka hoʻomaikaʻi ʻana i ka pono o ka hopena, a hiki i kēia mana ke hiki. e lyophilized.Eia hou, hiki iā ia ke hoʻokuʻu i kāna hana ma nā mahana kiʻekiʻe, no laila e hoʻopau i ka pono no kahi kaʻina hana hoʻokaʻawale.
Nā ʻāpana
| ʻāpana | HC5007A-01 | HC5007A-02 | HC5007A-03 |
| Bst DNA polymerase V2 (Glycerol-free) (32U/μL) | 0.05 mL | 0.25 mL | 2.5 mL |
| 10×HC Bst V2 Hoʻopaʻa | 1.5 mL | 2×1.5 mL | 3×10 mL |
| MgSO4 (100mM) | 1.5 mL | 2×1.5 mL | 2×10 mL |
Nā noi
1.LAMP isothermal amplification
2.Hoʻololi hoʻoneʻe hoʻokahi ke kaula DNA
3.Kiʻekiʻe GC gene sequencing
4.ʻO ke kaʻina hana DNA o ka pae nanogram.
Kūlana Waihona
ʻO ka halihali ma lalo o 0°C a mālama ʻia ma -25°C~-15°C.
Wehewehe Unite
Hoʻokahi ʻāpana i wehewehe ʻia ʻo ka nui o ka enzyme e hoʻokomo i 25 nmol o dNTP i loko o ka mea hiki ke hoʻoheheʻe ʻia i loko o 30 mau minuke ma 65°C.
LAMP Pane
| Nā ʻāpana | 25 μLPūnaehana |
| 10×HC Bst V2 Hoʻopaʻa | 2.5 μL |
| MgSO4 (100mM) | 1.5 μL |
| dNTP (10mM kēlā me kēia) | 3.5 μL |
| SYTO™ 16 ʻōmaʻomaʻo (25×)a | 1.0 μL |
| Huina muab | 6 μL |
| Bst DNA Polymerase V2 (Glycerol-free) (32 U/uL) | 0.25 μL |
| Papahana | × μL |
| ddH₂O | A hiki i ka 25 μL |
Nā memo:
1) a.SYTOTM 16 ʻOmaomao (25×): E like me nā pono hoʻokolohua, hiki ke hoʻohana ʻia nā mea pena ʻē aʻe i pani;
2) b.Hui mua: loaʻa ma ka hui ʻana i 20 µM FIP, 20 µM BIP, 2.5 µM F3, 2.5 µM B3, 5 µM LF, 5 µM LB a me nā puke ʻē aʻe.
Pane a me ke kūlana
1 × HC Bst V2 Buffer, aia ka mahana incubation ma waena o 60°C a me 65°C.
ʻAʻohe wela
80°C, 20min.
