ʻO ka mahanahana UNG

Loaʻa ka UNG (TS-UNG) ma ke ʻano recombinant ma E. coli.Hoʻopili ka enzyme i ka hoʻokuʻu ʻana o ka uracil manuahi mai ka uracil i loaʻa i ka DNA hoʻokahi a me ke kaula pālua a ʻaʻohe hana kūʻē iā RNA.Hoʻohālikelike ʻia me ka enzyme UNG maʻamau o E. coli gene origin, ʻoi aku ka ʻoi aku o ka TS-UNG enzyme i nā haʻahaʻa haʻahaʻa (20 ℃ ~ 37 ℃) a me ka maʻalahi o ka mahana a me ka maʻalahi ʻole (50 ℃), e pale ana i ka hoʻohaʻahaʻa ʻana o ka dUTP-containing amplification. nā huahana ma ka lumi wela e ke koena hana i hiki ke noho ma hope o ka hoʻopau ʻana o ka enzyme UNG maʻamau.No laila, ʻaʻole kūpono wale ka TS-UNG enzyme no ka hopena pale ʻana i ka PCR contamination, akā pili maikaʻi pū me ka papahana hoʻonui RT-PCR a hiki ke hoʻohana ʻia i ka pane pale ʻana i ka contamination RT-PCR.

Manaʻo Noi

Hoʻonui Kāohi ʻana i ka hoʻohaumia

Kūlana Waihona

-20°C no ka mālama ʻana i ka wā lōʻihi, pono e hui pū ʻia ma mua o ka hoʻohana ʻana, e pale i ka hoʻoheheʻe pinepine ʻana.

Paʻa mālama

20 mM Tris-HCl (pH 7.5) , 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, Stabilizer, 50% Glycerol.

Wehewehe Unite

ʻO ka nui o ka enzyme e pono ai e hoʻohaʻahaʻa i 1µg o ka DNA kaula hoʻokahi i loaʻa nā kumu dU i 1 hola ma 37°C he 1 ʻāpana hana (U).

Ka Mana Mana

1.ʻOi aku ka maʻemaʻe electrophoretic SDS-PAGE ma mua o 98%

2.ʻO ka hana hoʻohaʻahaʻa, ka hoʻomalu ʻana i ka puʻupuʻu, kūpaʻa

3.ʻAʻohe hana nuclease exogenous, ʻaʻohe exogenous endonuclease a exonuclease contamination.

Nā kuhikuhi

'Ōlelo Aʻo: a: Inā hoʻohana ʻia no qPCR/qRT-PCR, pono e hoʻohui ʻia ka ʻimi fluorescent i loko o ka ʻōnaehana pane.ʻO ka maʻamau, hiki ke hāʻawi i nā hopena maikaʻi i kahi ʻano kumu mua o 0.2 μM;i ka wā ʻilihune ka hana ʻana, hiki ke hoʻoponopono ʻia ke kumu kumu ma ka laulā o 0.2-1 μM.ʻO ka maʻamau, ʻoi aku ka maikaʻi o ka ʻimi noiʻi ma ka laulā o 0.1-0.3 μM.Hiki ke hana ʻia nā hoʻokolohua gradient concentration no ka ʻimi ʻana i ka hui maikaʻi loa o ka primer a me ka probe.

Nā memo

1.ʻO ka mahana hopena maikaʻi loa o ka TS-UNG enzyme he haʻahaʻa haʻahaʻa, a hiki ke hoʻonui ʻia i ka laulā o 20 ℃ ~ 37 ℃, hiki ke hoʻopaʻa ʻia ke ʻano o ka enzyme a me ka manawa pane i ka laulā o 0.1 ~ 0.5 U, 5 ~ 10 min;a hiki ke hoʻopau ʻia ka enzyme ma ke kaʻina o ka hoʻololi ʻana.

2.He kūpono ia no ka PCR a me ka RT-PCR e pale aku i ka haumia.

3.E hōʻalo i ka hoʻoheheʻe pinepine ʻana, a mai hōʻike i nā loli wela nui.

4.ʻO nā ʻano genes e hoʻonui ʻia he ʻokoʻa ka hoʻohana pono ʻana o ka dUTP a me ka naʻau i ka enzyme UNG, no laila, inā ʻo ka hoʻohana ʻana i ka ʻōnaehana UNG e alakaʻi i ka emi ʻana o ka ʻike ʻike, pono e hoʻoponopono ʻia ka ʻōnaehana hopena, inā makemake ʻoe i ke kākoʻo ʻenehana, e ʻoluʻolu e kelepona. ko makou hui.