Ultra Nuclease

ʻO UltraNuclease kahi genetically engineeredendonuclaese i loaʻa mai iā Serratia marcescens, hiki ke hoʻohaʻahaʻa i ka DNA a i ʻole RNA, ʻelua a i ʻole hoʻokahi stranded, linear a pōʻai paha ma lalo o kahi ākea ākea, hoʻohaʻahaʻa loa i nā waikawa nucleic i 5'-monophosphate oligonucleotides me 3-5base lōʻihi. .Ma hope o ka hoʻololi ʻana i ka genetic engineering, ua fermented, hōʻike ʻia, a hoʻomaʻemaʻe ʻia ka huahana ma Escherichia coli (E. coli), ka mea e hoʻemi ai i ka viscosity o ka supernatant cell a me ka cell lysate inscientific research, akā hoʻomaikaʻi pū i ka hoʻomaʻemaʻe pono a me ka noiʻi hana o ka protein.Hiki ke hoʻohana ʻia i ka gene therapy, ka hoʻomaʻemaʻe ʻana i ka maʻi virus, ka hana kano, ka protein a me ka polysaccharide pharmaceutical industry ma ke ʻano he mea hoʻokipa koena nucleic acid reagent.

Nā hiʻohiʻona huahana

Nā kūlana mālama

≤0°C ka lawe ʻana;-25~-15°C Ka waiho ʻana, 2 mau makahiki pono (hōʻalo i ka hoʻoheheʻe ʻana).

Wehewehe Unite

ʻO ka nui o ka enzyme i hoʻohana ʻia e hoʻololi i ka waiwai absorption o △A260 e 1.0 i loko o 30min ma 37 °C, pH 8.0, e like me ka digested 37μg salmon sperm DNA ma o ka ʻoki ʻana i loko o nā oligonucleotides, ua wehewehe ʻia ma ke ʻano he unit hana (U).

Ka hoomalu maikai

Koena Host-cell Protein: ELISA kit

•Protease Koena: 250KU/mL UltraNuclease reacted me substrate no 60min, aole hana i ikeia.

•Endotoxin maʻi bacteria: LAL-Test, Pharmacopoeia of the People's Republic of China Volume 4 (2020 Edition) gel limit test method.Nā Rula Nui (1143).

•ʻO ke kaumaha olaola: Pharmacopoeia of the People's Republic of Kina Volume 4 (2020 Edition)— General

Nā lula no ka ho'āʻo ʻana i ka maʻemaʻe (1101), PRC National Standard, GB 4789.2-2016.

•Metala kaumaha:ICP-AES, HJ776-2015.

Hana

Ua kāohi nui ʻia ka hana UltraNuclease i ka wā i ʻoi aku ka nui o ka SDS ma luna o 0.1% a i ʻole EDTA

ʻoi aku ka nui o ka ʻike ma luna o 1mM. ʻAʻohe hopena o ka Surfactant Triton X- 100, Ma waena o 20 a me Tween 80 i ka nuclease.

nā waiwai i ka wā i emi iho ai ka 1.5%.

 Hoʻohana a me ka hoʻohana ʻana

• Wehe i ka waika nucleic exogenous mai nā huahana lāʻau lapaʻau, e hoʻemi i ka pilikia o ke koena nucleic acid a hoʻomaikaʻi i ka palekana o ka huahana.

• E ho'ēmi i ka viscosity o ka wai hānai i hanaʻia e ka nucleic acid, e hoʻopōkole i ka manawa hana a hoʻonui i ka hua protein.

• Wehe i ka waika nucleic i wahī i ka ʻāpana (virus, kino hoʻohui, a me nā mea ʻē aʻe), he mea kūpono

i ka hoʻokuʻu ʻana a me ka hoʻomaʻemaʻe ʻana i ka ʻāpana.

• Hiki ke hoʻomaikaʻi i ka hoʻonā ʻana a me ka hoʻihoʻi ʻana o ka hāpana no ka chromatography kolamu, electrophoresis a me ka nānā ʻana i ka blotting.

• I loko o ka gene therapy, wehe 'ia ka nucleic acid no ka loa'a 'ana o nā ma'i ma'ema'e e pili ana i ka adeno.