Alkaline Phospatase(ALP)

Loaʻa ka Alkaline Phosphatase mai kahi recombinant E. coli strain e lawe ana i ka gene TAB5.Hoʻopili ka enzyme i ka dephosphorylation o 5' a me 3' hopena o DNA a me RNA phosphomonoesters.Eia kekahi, hoʻoheheʻe ia i ka ribose, a me ka deoxyribonucleoside triphosphates (NTPs a me dNTPs).Hana ʻo TAB5 Alkaline Phosphatase ma nā 5' puka, 5' hope a me ka pōpō.Hiki ke hoʻohana ʻia ka Phosphatase i nā noi biology molecular, e like me ka cloning a i ʻole ka hoʻopaʻa ʻana i ka hopena e hoʻoneʻe i nā hopena phosphorylated o DNA a i ʻole RNA.I nā hoʻokolohua cloning, pale ka dephosphorylation i ka linearized plasmid DNA mai ka hoʻopili ponoʻī.Hiki iā ia ke hoʻohaʻahaʻa i nā dNTP i hoʻohui ʻole ʻia i nā hopena PCR e hoʻomākaukau i kahi mamana no ka hoʻopili ʻana i ka DNA.Hoʻopau ʻole ʻia ka enzyme ma ka hoʻomehana ʻana ma 70 ° C no 5 mau minuke, a laila e hoʻoneʻe ʻia ka phosphatase ma mua o ka ligation a i ʻole ka hoʻopau ʻana i ka lepili ʻaʻole pono.