DNase I (Rnase Free) (2u/ul)
Helu popoki: HC4007B
ʻO DNase I kahi endonuclease hiki ke ʻeli i ka DNA i hoʻokahi a i ʻole pālua.Hiki iā ia ke hoʻokahe i nā paʻa phosphodiester e hana i nā mono-a me oligodeoxynucleotides i loaʻa kahi hui 5'-phosphate a me kahi hui 3'-OH.ʻO ka pae pH hana maikaʻi loa o DNase I ʻo 7-8.ʻO ka hana o DNase I pili iā Ca2+a hiki ke ho'ā 'ia e divalent metala ion e like me CO2, Mn2+, Zn2+, etc. Ma ke alo o Mg2+, DNase Hiki iaʻu ke hoʻokaʻawale maʻamau i kekahi kahua o ka DNA kaulalua;Oiai ma ke alo o Mn2+, DNase Hiki iaʻu ke hoʻokaʻawale i ka DNA i kau ʻia ma ka pae hoʻokahi, e hana ana i nā wēlau pōpō a i ʻole nā ʻaoʻao pili me 1-2 nucleotides e puka mai ana.Hiki ke hoʻohana ʻia no ka hana ʻana i nā laʻana RNA like ʻole.
Nā ʻāpana
| inoa | 1KU | 5KU |
| DNaseI hui hou (RNase-ʻole) | 500 μL | 5 ×500 μL |
| DNase I Hoʻopaʻa Paʻa (10×) | 1 mL | 5 × 1mL |
Nā kūlana mālama
Pono e mālama ʻia kēia huahana ma -25 ~ -15 ℃ no 2 mau makahiki.E ʻoluʻolu e pale i ka hoʻoheheʻe hou ʻana.
Nā kuhikuhi
Hoʻohana ʻia i ka wehe ʻana i ka DNA mai nā laʻana RNA no ke kuhikuhi wale ʻana.
1. E ʻoluʻolu e hoʻohana i nā paipu centrifuge RNase-free a me nā ʻōlelo aʻoaʻo pipette e hoʻomākaukau ai i kēia ʻōnaehana pane:
| Nā ʻāpana | Volume (μL) |
| DNase I Hoʻopaʻa Paʻa (10×) | 1 |
| DNasel hui hou (RNase-ʻole) | 1 |
| RNA | X |
| RNase-noa ddH2O | A hiki i ka 10 |
2. Penei ka hopena o ka hopena: 37 ℃, ma hope o 15-30 mins, e hoʻohui i ka hopena hope o 2.5 mM EDTA solution a hui maikaʻi, a laila 65 ℃ no 10 mins.Hiki ke hoʻohana ʻia ka laʻana i hoʻoponopono ʻia no nā hoʻokolohua RT-PCR a i ʻole RT-qPCR, etc.
Nā memo
1. ʻO DNase l ka mea maʻalahi i ka denaturation kino;I ka hui ʻana, hoʻohuli mālie i ka paipu hoʻāʻo ae lulu maikai, mai lulu ikaika.
2. Pono e mālama ʻia ka enzyme i loko o ka pahu hau a i ʻole ma ka ʻauʻau hau ke hoʻohana ʻia, a pono e mālama ʻia ma -20 ℃ ma hope koke o ka hoʻohana ʻana.
3. No ka noiʻi wale nō kēia huahana.
4. E ʻoluʻolu e hana me nā ʻaʻahu lab a me nā mīkina lima hoʻopau, no kou palekana.
