Laʻana Hoʻokuʻu Reagent
ʻO Sample Release Reagent no nā hiʻohiʻona diagnostic POCT molekula.No nā ʻōnaehana ʻelua o ka LAMP hoʻonui pololei a me ka PCR hoʻonui pololei, ʻaʻohe pono no ka unuhi ʻana i ka waikawa nucleic.Hiki ke hoʻonui pono ʻia ka lysate crude o ka hāpana, hiki ke ʻike pololei ʻia ka gene target, hiki ke hoʻopōkole ʻia ka manawa ʻike hōʻike, i kūpono loa i nā koi noi o POCT molecular.He kūpono ia no nā ʻōpala ihu, nā ʻāʻī a me nā ʻano laʻana ʻē aʻe.Hiki ke hoʻohana pololei ʻia nā laʻana i hoʻopaʻa ʻia no ka PCR a i ʻole ka ʻike LAMP fluorescence manawa maoli, a hiki ke hoʻokō ʻia nā hopena like me nā ʻano hoʻoheheʻe maʻamau me ka ʻole o nā hana unuhi nucleic acid.
Nā Kūlana Waihona
Lawe a mālama i ka lumi wela.
Ka hoomalu maikai
ʻIke hana - quantitative qPCR: Ua hoʻonui ʻia ka 800μl Sample Release Reagent system.
me 1000 kope Novel Pseudovirus, hoʻokahi laʻana nasal swab, ka hopena o nā pihi hoʻonui like aΔCt waiwai i loko o ± 0.5 Ct.
Kaʻina hana hoʻokolohuares
1. E lawe i 800 μl Sample Release Reagent a hohola i ka solution lysis i loko o 1.5 mL sampling tube
2. E lawe i ka nasal swab a i ka pu'u paha me ka swab; Nasal swab sampling kaʻina hana: lawe i ka sterile swab a hoʻokomo i loko o nā puka ihu, neʻe mālie a hiki i kahi 1.5 knm hohonu, e hoʻololi mālie i nā manawa 4 e kū'ē i ka nasal mucosa no 15 kekona. , A laila e hana hou i ka hana like ma ka lua ihu ʻē aʻe me ka swab.Throat swab sampling kaʻina hana: lawe i ka sterile swab a me ka mālie, holoi koke i ka pharyngeal tonsils a me ka pā pharyngeal hope 3 manawa.
3.E hoʻokomo koke i ka swab i loko o ka paipu hōʻailona.Pono e hoʻohuli ʻia ke poʻo swab a hui pū ʻia i loko o ka wai hoʻopaʻa no ka liʻiliʻi loa he 30 kekona e hōʻoia i ka hoʻoheheʻe ʻia ʻana o ka hāpana i loko o ka paipu laʻana.
4. Incubation ma ka lumi wela (20 ~ 25 ℃) no 1min, ua pau ka hoomakaukau ana o ka lysis buffer.
5. Ua kūpono nā ʻōnaehana ʻelua 25μl RT-PCR a me RT-LAMP me 10μl nui o ka hoʻohui template no nā hoʻokolohua ʻike.
Nā memo
1. ʻO ka liʻiliʻi liʻiliʻi o ka hāpana pololei lysate e pili ana i kahi swab hoʻokahi hiki ke hoʻololi i 400μl, hiki ke hoʻololi ʻia e like me nā pono hoʻāʻo.
2. I ka manawa i hoʻoponopono ʻia ai ka hāpana e ka hāpana hoʻokuʻu reagent, ʻoi aku ka maikaʻi o ka hana ʻana i ka pae aʻe o ka hoʻāʻo i ka wā hiki, ʻoi aku ka liʻiliʻi o ka manawa kali ma mua o 1 hola.
3. ʻO ka pH o ka lysate hāpana he acidic, a pono e loaʻa i ka ʻōnaehana ʻike i kahi pale.He kūpono ia no ka hapa nui o ka PCR, RT-PCR, a me LAMP fluorescence detection with pH buffer, akā ʻaʻole kūpono no ka LAMP colorimetric detection me ka ʻole o ka pale.
