ʻO ka pahu hōʻike hōʻike ʻana o Ultra Nuclease (ELISA)
wehewehe
ʻO kēia kit Ultra Nuclease ELISA he one kahi e hana ʻia ai ʻo ELISA ma kahi ʻano microplate.Hoʻokomo ʻia ka laʻana i loaʻa i ka endonuclease i loko o nā pūnāwai microtiter plate i uhi mua ʻia me ka anti-endonuclease capture antibody.Ma hope o ka incubation a me kahi holoi holoi kahi e wehe ʻia ai nā mea i hoʻopaʻa ʻole ʻia, hoʻohui ʻia kahi enzyme-conjugated, anti-endonuclease detector antibody.ʻO ka hopena o ka immunological ka hopena i ka hoʻokumu ʻana i kahi paʻi sandwich o ka antibody-endonuclease-enzyme i kapa ʻia i ka antibody.Ma hope o ka holoi ʻana, hoʻohui ʻia kahi hopena substrate i nā pūnāwai a hana ʻia, e hopena i ka ulu ʻana o ke kala.Ua ana ʻia ka mānoanoa opitika i ka photometric a ua kūlike me ka
analyte concentrates i loko o nā pūnāwai.Hiki ke helu ʻia ka manaʻo endonuclease i nā laʻana i ʻike ʻole ʻia ma muli o ka pihi maʻamau e pili ana.
Hoʻolālā Kimia
Wehewehe Unite
ʻO ka nui o ka enzyme i hoʻohana ʻia e hoʻololi i ka waiwai absorption o △A260 e 1.0 i loko o 30min
ma 37 °C, pH 8.0, e like me ka digested 37μg salmon sperm DNA ma ka ʻoki ʻana i loko o nā oligonucleotides, ua wehewehe ʻia he ʻano hana.
Hoʻohana a me ka hoʻohana ʻana
• Wehe i ka waika nucleic exogenous mai nā huahana lāʻau lapaʻau, e hoʻemi i ka pilikia o ke koena nucleic acid a hoʻomaikaʻi i ka palekana o ka huahana.
• E ho'ēmi i ka viscosity o ka wai hānai i hanaʻia e ka nucleic acid, e hoʻopōkole i ka manawa hana a hoʻonui i ka hua protein.
• Wehe i ka waika nucleic i wili ʻia (virus, inclusion body, etc.), i kūpono i ka hoʻokuʻu ʻana a me ka hoʻomaʻemaʻe ʻana i ka ʻāpana.
• Hiki ke hoʻomaikaʻi i ka hoʻonā ʻana a me ka hoʻihoʻi ʻana o ka hāpana no ka chromatography kolamu, electrophoresis a me ka nānā ʻana i ka blotting.
• I loko o ka gene therapy, wehe 'ia ka nucleic acid no ka loa'a 'ana o nā ma'i ma'ema'e e pili ana i ka adeno.
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| palena haʻahaʻa o ka ʻike | 0.6 ng/mL |
| palena haʻahaʻa o ka nui | 0.2 ng/mL |
| pololei | Intra assay CV≤10% |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:Hoʻouna ʻia ma lalo o 0 °C
Waihona:E kūʻai ma -2-8°C, wehe ʻia ka reagent stable no 6 pule
Manaʻo ʻia e hoʻāʻo hou i ke ola:1 makahiki
