Ultra Nuclease
wehewehe
ʻO UltraNuclease kahi endonuclaese genetically engineered i loaʻa mai iā Serratia marcescens, hiki ke hoʻohaʻahaʻa i ka DNA a i ʻole RNA, ʻelua a i ʻole hoʻokahi stranded, linear a i ʻole pōʻai ma lalo o kahi ākea ākea, hoʻohaʻahaʻa loa i nā waikawa nucleic i 5'-monophosphate oligonucleotides me 3-5 lōʻihi kumu.
Ma hope o ka genetic engineering hoʻololi, ua fermented ka huahana, hōʻike, a hoʻomaʻemaʻe i loko o Escherichia coli (E. coli), ka mea e hoemi i ka viscosity o cell supernatant a me cell lysate i ka 'epekema noiʻi, akā, e hoʻomaikaʻi i ka hoʻomaʻemaʻe pono a me ka hana noiʻi o ka protein.Hiki ke hoʻohana ʻia i ka gene therapy, ka hoʻomaʻemaʻe ʻana i ka maʻi virus, ka hana ʻana i ke kano, ka protein a me ka polysaccharide pharmaceutical industry ma ke ʻano he host reagent nucleic acid reagent.
Hoʻolālā Kimia
Wehewehe Unite
ʻO ka nui o ka enzyme i hoʻohana ʻia e hoʻololi i ka waiwai absorption o △A260 e 1.0 i loko o 30min ma 37 °C, pH 8.0, e like me ka digested 37μg salmon sperm DNA ma o ka ʻoki ʻana i nā oligonucleotides, ua wehewehe ʻia he ʻāpana hana.
Hoʻohana a me ka hoʻohana ʻana
• Wehe i ka waika nucleic exogenous mai nā huahana lāʻau lapaʻau, e hoʻemi i ka pilikia o ke koena nucleic acid a hoʻomaikaʻi i ka palekana o ka huahana.
• E ho'ēmi i ka viscosity o ka wai hānai i hanaʻia e ka nucleic acid, e hoʻopōkole i ka manawa hana a hoʻonui i ka hua protein.
• Wehe i ka waika nucleic i wili ʻia (virus, inclusion body, etc.), i kūpono i ka hoʻokuʻu ʻana a me ka hoʻomaʻemaʻe ʻana i ka ʻāpana.
• Hiki ke hoʻomaikaʻi i ka hoʻonā ʻana a me ka hoʻihoʻi ʻana o ka hāpana no ka chromatography kolamu, electrophoresis a me ka nānā ʻana i ka blotting.
• I loko o ka gene therapy, wehe 'ia ka nucleic acid no ka loa'a 'ana o nā ma'i ma'ema'e e pili ana i ka adeno.
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| wehewehe | Akaka a kala ole |
| Ka hana | ≥ 250 U/ul |
| Hana kūikawā | ≥1.1*106U/mg |
| Maʻemaʻe （SDS-PAGE） | ≥ 99.0% |
| Nā mea pale | ʻAʻohe mea i ʻike ʻia |
| ʻO ke kaumaha kino | ＜10 cfu/100,000U |
| Endotoxine(LAL-hoʻāʻo) | ＜ 0.25EU/1,000U |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:Hoʻouna ʻia ma lalo o 0 °C
Waihona:E mālama i ka -25~-15°C
Manaʻo ʻia e hoʻāʻo hou i ke ola:2 makahiki (hōʻalo i ka hau hau)
