ELEISA KIT no Trypsin

Hoʻohana pinepine ʻia ʻo Recombinant Trypsin i ka hana biopharmaceutical-i ka wā hoʻomākaukau cell a i ʻole no ka hoʻololi ʻana a me ka hoʻōla ʻana i nā huahana.He pilikia ka Trypsin a no laila pono e wehe ʻia ma mua o ka hoʻokuʻu ʻia ʻana o ka huahana.ʻO kēia Sandwich Kit no ke ana nui o Residual Trypsin i loko o ka supernatant moʻomeheu cell a me nā kaʻina hana ʻē aʻe i ka hana biopharmaceutical ke hoʻohana ʻia ʻo Trypsin.

ʻO kēia pahu kahi Enzyme-Linked Immunosorbent Assay (ELISA).Ua uhi mua ʻia ka pā me ka Porcine trypsin antibody.Hoʻohui ʻia ʻo Trypsin i loko o ka laʻana a hoʻopaʻa ʻia i nā antibodies i uhi ʻia ma nā pūnāwai.A laila hoʻohui ʻia ka antibody Porcine trypsin biotinylated a hoʻopaʻa iā trypsin i loko o ka hāpana.Ma hope o ka holoi ʻana, hoʻohui ʻia ʻo HRP-Streptavidin a hoʻopaʻa i ka antibody trypsin Biotinylated.Ma hope o ka incubation unbound HRP-Streptavidin holoi ʻia.A laila hoʻohui ʻia ka hopena substrate TMB a hoʻopaʻa ʻia e HRP e hana i kahi huahana kala polū i hoʻololi ʻia i melemele ma hope o ka hoʻohui ʻana i ka hopena hoʻomaha acidic.Hoʻohālikelike ka nui o ka melemele i ka nui o trypsin
laʻana i hopu ʻia ma ka pā.Ana ʻia ka absorbance ma 450 nm.