Glycerol Kinase(GK)
wehewehe
No ka ʻohana FGGY kinase ka pūmua i hoʻopaʻa ʻia e kēia gene.ʻO kēia protein kahi enzyme nui i ka hoʻoponopono ʻana i ka glycerol uptake a me ka metabolism.Hoʻopili ia i ka phosphorylation o ka glycerol e ATP, e hāʻawi ana i ka ADP a me ka glycerol-3-phosphate.Hoʻopili ʻia nā hoʻololi ʻana o kēia ʻano me ka glycerol kinase deficiency (GKD).Ua ʻike ʻia nā ʻokoʻa transcript i hoʻopili ʻia no kēia gene.
Hoʻohana ʻia kēia enzyme no nā hoʻokolohua diagnostic no ka hoʻoholo ʻana i nā triglycerides me Glycerol-3-phosphate Oxidase.
Hoʻolālā Kimia
Kuʻuna Pane
Glycerol + ATP→ Glycerol -3- phosphate + ADP
Hōʻike
| Nā mea ho'āʻo | Nā kikoʻī |
| wehewehe | Keokeo a melemele iki amorphous pauka, lyophilized |
| Ka hana | ≥15U/mg |
| Maʻemaʻe (SDS-PAGE) | ≥90% |
| Solubility (10mg pauka/ml) | Akaka |
| Catalase | ≤0.001% |
| Glucose oxidase | ≤0.01% |
| Uricase | ≤0.01% |
| ATPase | ≤0.005% |
| Hexokinase | ≤0.01% |
Ka lawe ʻana a me ka mālama ʻana
Kaʻahele:Hoʻouna ʻia ma lalo o -15°C
Waihona :E kūʻai ma -20°C(Loaʻa), 2-8°C(Pokoʻa)
Manaʻo ʻia e hoʻāʻo houola:18 mahina
huahana pili
E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou
